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> Mouse Monoclonal Anti-Keratin Pan, [Clone Lu-5]

Mouse Monoclonal Anti-Keratin Pan, [Clone Lu-5]

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Referencia 610145

embalaje : 50µg

Marca : Progen

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Teléfono : +1 850 650 7790

Conformational epitope
Highly published

anti-Keratin Pan mouse monoclonal, Lu-5, lyophilized, purified

anti-Keratin Pan mouse monoclonal, Lu-5, lyophilized, purified
anti-Keratin Pan mouse monoclonal, Lu-5, lyophilized, purified
anti-Keratin Pan mouse monoclonal, Lu-5, lyophilized, purified
anti-Keratin Pan mouse monoclonal, Lu-5, lyophilized, purified
Product: anti-Keratin Pan mouse monoclonal, Lu-5, lyophilized, purified
Art.No.: 610145

Quantity 50 µg
Antibody Type Monoclonal
Host Mouse
Isotype IgG1
Conjugate Unconjugated
Application ICC/IF, IHC
Purification Affinity chromatography
Reactivity Bovine, Chicken, Human, Mouse, Pleurodeles, Rat, Snake, Xenopus
Storage before reconstitution 2-8°C
Storage after reconstitution Up to 3 months at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/thaw cycles
Intended use Research use only
Clone Lu-5
Immunogen Cells of lung cancer cell line A549 and A2182
Concentration 50 µg/ml after reconstitution with 1 ml dist. water
Formulation Lyophilized; reconstitute in 1 ml dist. water (final solution contains 0.09% sodium azide, 0.5% BSA in PBS buffer, pH 7.4)
Tested applications Tested dilutions
Immunohistochemistry (IHC) - frozen Assay dependent
Immunohistochemistry (IHC) - paraffin 1:10-1:20 (protease treatment and/or microwave treatment recommended)
Western Blot (WB) Not applicable

Lu-5 represents an excellent marker for all types of epithelia. Ideal marker for immunolocalization studies on frozen and paraffin embedded tissue. Useful for distinguishing carcinomas from non-epithelial tumors.
Tumors specifically detected: all epithelium-derived neoplasms.

Polypeptide reacting: Most keratins present in human epithelia (including keratins - formerly also designated cytokeratins -K1, K5, K6, K14, K18, K19) and most acidic (K31 - KK38) and basic (K81 - K86) hair keratins from human hair follicle. The antibody specifically detects a conformational epitope common to most keratins, but not to other intermediate filament polypeptides.

Reactivity on cultured cell lines: MCF-7, PLC, A-431, HeLa; PtK2, A6 (XLKE).

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