600217 | Mouse TNF-alpha Clinisciences

Recombinant Active Protein

Cat. No.

600217

Unit

0.02 mg

Description

Tumor necrosis factor-alpha (TNF-alpha) is a cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by mactrophages and can induce cell death of certain cell lines. It is a potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia. Recombinant mouse TNF-alpha produced in S. lividans is a single non-glycosylated polypeptide chain containing 156 amino acids with a MW of 17301.32 Da.

Spec Container

Datasheet

Format

Each vial contains 20 ug protein lyophilized in 50 mM Tris-HCl, pH8.0, 20 mM NaCl. Reconstitute with 0.2 ml sterile deionized water for a final concentration of 0.1 mg/ml.

Alternate Names

tumor necrosis; TNF- alpha; tumor necrosis factor ligand superfamily member 2; TNF-a; cachectin; tumor necrosis factor, membrane form; tumor necrosis factor, soluble form

Accession No.

P06804

17301 Da

Sequence

MLRSSSQNSS DKPVAHVVAN HQVEEQLEWL SQRANALLAN GMDLKDNQLV VPADGLYLVY SQVLFKGQGC PDYVLLTHTV SRFAISYQEK VNLLSAVKSP CPKDTPEGAE LKPWYEPIYL GGVFQLEKGD QLSAEVNLPK YLDFAESGQV YFGVIAL

Source

Streptomyces lividans. Endotoxin level as measured by LAL is <0.01ng/ug or <0.1EU/ug.

Purity

Purity > 98% as determined by RP-HPLC and reducing and non-reducing SDS-PAGE. Protein Content determined by UV spectroscopy at 280 nm and analysis by RP-HPLC calibrated against a known standard. Quantitation on SDS-PAGE against a known standard.

Application Notes

rmTNF-alpha is fully biologically active when compared to standard. The ED50 as determined by the cytolysis of mouse L929 cells in the presence of Actinomycin D is less then 0.05ng/ml, corresponding to a Specific Activity of 9x108 IU/mg.

Storage

Store at -20°C. Product is guaranteed one year from the date of shipment. Following reconstitution, store at -20°C. We recommend to add a carrier protein (0.1% HSA or BSA) for long term storage.

Images

For research use only, not for diagnostic or therapeutic procedures.

Mouse TNF-alpha

Referencia 600217

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Marca : Abbiotec

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Spec Container