MUC5AC (Apomucin Major Airway GlycoProtein, Mucin 5 subtype AC tracheobronchial, Mucin 5 Subtypes A And C, Mucin 5AC oligomeric mucus/gel forming, Tracheobronchial Mucin, TBM, Mucin 5AC, Gastric Mucin) (APC)

Antibody to gastric mucin M1 reacts with the gastric epithelium of normal human gastrointestinal tract as well as with the precancerous and cancerous colon but not with normal adult colon. It also reacts with fetal colonic mucosa. Resurgence of gastric mucin reactivity during colonic carcinogenesis is due to re-expression of the peptide core of gastric (or fetal colonic) mucins.||Applications: |Suitable for use in Immunofluorescence, Flow Cytometry, FLISA and Immunohistochemistry. Other applications not tested.||Recommended Dilution:|Immunohistochemistry (Formalin-fixed): Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris, 1mM EDTA, pH 9.0, for 45 minutes followed by cooling at RT for 20 minutes)|Optimal dilutions to be determined by the researcher. ||Positive Control:|Human, adult normal and gastric carcinoma |Human, adult colon carcinoma||Storage and Stability:|Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.| |Note: Applications are based on unconjugated antibody.