NMDA 1 Receptor (NMDA R1, N-methyl-D-aspartate) (HRP)

Referencia N2904-35-HRP-100ul

embalaje : 100ul

Marca : US Biological

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N2904-35-HRP NMDA 1 Receptor (NMDA R1, N-methyl-D-aspartate) (HRP)

Clone Type
Polyclonal
Host
mouse
Swiss Prot
Q05586
Isotype
IgG2a
Grade
Affinity Purified
Applications
E IC IHC RIA
Crossreactivity
Hu Mk Rt Xe
Accession #
NM_000832.5
Shipping Temp
Blue Ice
Storage Temp
-20°C

The ion channels activated by glutamate are divided into two classes. Those that are sensitive to N-methyl-D-aspartate (NMDA) are designated NMDA receptors (NMDAR) while those activated by kainate and a-amino-3-hydroxy-5-methyl- 4-isoxalone propionic acid (AMPA) are known as kainate/AMPA receptors (K/AMPAR). NMDA receptors are among the most studied receptors in neuroscience. They are involved in neuronal cell development and plasticity, a cellular correlate for learning. NMDA receptors are implicated in several disorders of the central nervous system including epilepsy and ischemic neuronal cell death. NMDA receptors appear to be a target for ethanol at physiological concentrations which may play a significant role in alcoholism.||Applications:|Suitable for use in Immunohistochemistry, Immunocytochemistry, Light and Electron Microscopy, ELISA and RIA. Other applications have not been tested.||Recommended Dilution:|Immunohistochemistry (Paraffin): Use standard deparaffinization techniques on tissue specimens. Pretreat tissues using a citrate buffer, pH 6.0 and high heat epitope retrieval techniques. Do not allow tissues to dry out during the staining procedure.|Optimal dilution determined by the researcher.||Immunohistochemistry Control:|rat brain and spinal cord tissue||Storage and Stability:|Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.||Note: Applications are based on unconjugated antibody.

Applications
Product Type: Mab|Isotype: IgG2a|Clone No: 2Q187 (54.1)|Host: mouse|Concentration: As Reported |Form: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with horseradish peroxidase (HRP).|Purity: Purified by Protein A affinity chromatography.|Immunogen: Recombinant fusion protein containing GST and NMDAR1|(aa 660-811). Epitope: extracellular loop between transmembrane regions III and IV.|Specificity: Recognizes an epitope between amino acids 660-811 of the NMDAR1 receptor. Shows no crossreactivity with NMDAR2, NMDAR3, NMDAR4 or NMDAR5. Species Crossreactivity: human, monkey, rat and Xenopus||Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Immunogen
Recombinant fusion protein containing GST and NMDAR1|(aa 660-811). Epitope: extracellular loop between transmembrane regions III and IV.
Form
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with horseradish peroxidase (HRP).
Purity
Purified by Protein A affinity chromatography.
Specificity
Recognizes an epitope between amino acids 660-811 of the NMDAR1 receptor. Shows no crossreactivity with NMDAR2, NMDAR3, NMDAR4 or NMDAR5. Species Crossreactivity: human, monkey, rat and Xenopus
References
1. J. Neuroscience (1994) 14:3603-3619. 2. J. Neuroscience (1995) 15:2707-2719. 3. J. Neuroscience (1996) 16:6830-6838. 4. Naskar, R., et al., Invest. Ophthalmol. Vis. Sci. (2000) 41:1940-1944. 5. Menna, Kumari, J. Biological Chemistry (2001) 276:29764-29771.