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> Octaethylene glycol monododecyl ether [3055-98-9]

Octaethylene glycol monododecyl ether [3055-98-9]

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Octaethylene glycol monododecyl ether (C12E8) is an non-ionic detergent that can be used for membrane protein extraction. Octaethylene glycol monododecyl ether can solubilize the viral membrane of intact influenza virus.

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Octaethylene glycol monododecyl ether Estructura química

Octaethylene glycol monododecyl ether Estructura química

No. CAS : 3055-98-9

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Based on 1 publication(s) in Google Scholar

Descripciòn

Octaethylene glycol monododecyl ether (C12E8) is an non-ionic detergent that can be used for membrane protein extraction. Octaethylene glycol monododecyl ether can solubilize the viral membrane of intact influenza virus[1][2].

Cellular Effect
Cell Line Type Value Description References
MOLT-4 IC50
29 μM
Compound: HL-8
Growth inhibition of HIV1 3B infected human MOLT4 cells after 5 days by MTT assay
Growth inhibition of HIV1 3B infected human MOLT4 cells after 5 days by MTT assay
[PMID: 18672367]
MOLT-4 IC50
59 μM
Compound: HL-8
Growth inhibition of human MOLT4 cells after 5 days by MTT assay
Growth inhibition of human MOLT4 cells after 5 days by MTT assay
[PMID: 18672367]
In Vitro

Octaethylene glycol monododecyl ether (C12E8) has been shown to solubilize the viral membrane of intact influenza virus. The mechanism of solubilization of the viral membrane is based on the accumulation of C12E8 molecules outside the membrane until the formation of micelles leading to the extraction of membrane constituents[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Octaethylene glycol monododecyl ether Related Antibodies
Peso molecular

538.75

Fòrmula

C28H58O9
No. CAS

3055-98-9
Appearance

Liquid (Density: 0.984～0.992 g/cm3)

Color

Colorless to light yellow

SMILES

CCCCCCCCCCCCOCCOCCOCCOCCOCCOCCOCCOCCO
Envío

Room temperature in continental US; may vary elsewhere.
Almacenamiento
Pure form -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvente y solubilidad
In Vitro: 

DMSO : 100 mg/mL (185.61 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.8561 mL 9.2807 mL 18.5615 mL
5 mM 0.3712 mL 1.8561 mL 3.7123 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Calculadora de molaridad

  • Calculadora de dilución

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.5 mg/mL (4.64 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 2.5 mg/mL (4.64 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Pureza y Documentación

Purity: ≥98.0%

SDS (312 KB)

COA (242 KB) HNMR (265 KB)

Instrucciones de manejo (2659 KB)
Referencias

  • [1]. Véronique Nardello-Rataj, et al. Aqueous solutions of didecyldimethylammonium chloride and octaethylene glycol monododecyl ether: Toward synergistic formulations against enveloped viruses. Int J Pharm. 2016 Sep 10;511(1):550-559.  [Content Brief]

    [2]. C Carnero Ruiz, et al. Characterization of mixed non-ionic surfactants n-octyl-β-D-thioglucoside and octaethylene-glycol monododecyl ether: micellization and microstructure. J Colloid Interface Sci. 2011 Sep 1;361(1):178-85.  [Content Brief]

  • [1]. Véronique Nardello-Rataj, et al. Aqueous solutions of didecyldimethylammonium chloride and octaethylene glycol monododecyl ether: Toward synergistic formulations against enveloped viruses. Int J Pharm. 2016 Sep 10;511(1):550-559.

    [2]. C Carnero Ruiz, et al. Characterization of mixed non-ionic surfactants n-octyl-β-D-thioglucoside and octaethylene-glycol monododecyl ether: micellization and microstructure. J Colloid Interface Sci. 2011 Sep 1;361(1):178-85.

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 1.8561 mL 9.2807 mL 18.5615 mL 46.4037 mL
5 mM 0.3712 mL 1.8561 mL 3.7123 mL 9.2807 mL
10 mM 0.1856 mL 0.9281 mL 1.8561 mL 4.6404 mL
15 mM 0.1237 mL 0.6187 mL 1.2374 mL 3.0936 mL
20 mM 0.0928 mL 0.4640 mL 0.9281 mL 2.3202 mL
25 mM 0.0742 mL 0.3712 mL 0.7425 mL 1.8561 mL
30 mM 0.0619 mL 0.3094 mL 0.6187 mL 1.5468 mL
40 mM 0.0464 mL 0.2320 mL 0.4640 mL 1.1601 mL
50 mM 0.0371 mL 0.1856 mL 0.3712 mL 0.9281 mL
60 mM 0.0309 mL 0.1547 mL 0.3094 mL 0.7734 mL
80 mM 0.0232 mL 0.1160 mL 0.2320 mL 0.5800 mL
100 mM 0.0186 mL 0.0928 mL 0.1856 mL 0.4640 mL
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Octaethylene glycol monododecyl ether Related Classifications

  • Anti-infection
  • Influenza Virus
Help & FAQs

Keywords:

Octaethylene glycol monododecyl ether3055-98-9C12E8Influenza Virusnon-ionicdetergentmembraneproteininfluenzavirusInhibitorinhibitorinhibit

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