Phenylalanine Ammonia Lyase (PAL) Activity Assay Kit

Referencia E-BC-K522-S-100

embalaje : 100Assays

Marca : Elabscience

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Teléfono : +1 850 650 7790

Detection Principle Phenylalanine ammonia lyase (PAL) can catalyze L-phenylalanine to produce trans-cinnamic acid and ammonia, and trans-cinnamic acid has the maximum absorption peak at 290 nm. PAL activity can be calculated by measuring the increase of OD value at 290 nm.
Synonyms PAL
Sample Type Serum,Plasma,Animal tissue,Cell
Detection Method Colorimetric method
Detection Instrument Spectrophotometer (290 nm)
Research Area Plant Physiology Series
Storage This product can be stored at 2-8°C for 12 months with shading light.
Valid Period 12 months
Sensitivity 0.78 U/g tissue
Detection Range 0.78-156 U/g tissue
Precision inter-assay CV: 4.6% | intra-assay CV: 3.1%
Assay Time 1 h 10 min

The recommended dilution factor for different samples is as follows (for reference only):

Sample Type Dilution Factor
10% Epipremnum aureum tissue homogenate 1
10% Carrot tissue homogenate 1
10% Green pepper tissue homogenate 1
10% Corn grain tissue homogenate 1

The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.

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