Phosphatase Inhibitor Cocktail II (100× DMSO) [C0003]
Referencia C0003-1mLx10
embalaje : 1mLx10
Marca : TargetMol
Contact local distributor :
Teléfono : +1 850 650 7790
Customer service consultation
Phosphatase Inhibitor Cocktail II (100× DMSO)
Catalog No. C0003
It is easy for the proteins to be degraded or dephosphorylated during in vitro extraction procedure, which could cause an inaccurate result of protein expression detection. Therefore, adding protease inhibitors or phosphatase inhibitors into the extracts would be an effective method to prevent degradation and dephosphorylation of protein.
The phosphatase inhibitor mixture can effectively inhibit the dephosphorylation of common phosphatases on proteins and maintain the original phosphorylation state of proteins.
All products from TargetMol are for Research Use Only. Not for Human or Veterinary or Therapeutic Use.The phosphatase inhibitor mixture can effectively inhibit the dephosphorylation of common phosphatases on proteins and maintain the original phosphorylation state of proteins.
| Pack Size | |
|---|---|
| 1 mL | |
| 1 mL * 10 | |
| 1 mL * 50 | |
| 1 mL * 100 |
Composition
| Ingredient | CAS | MW | Concentration (100x) | Target | Type |
|---|---|---|---|---|---|
| (-)-p-Bromotetramisole oxalate | 62284-79-1 | 373.22 | 2.5 mM | Alkaline phosphatases | Irreversible |
| Cantharidin | 56-25-7 | 196.2 | 500 μM | Ser/Thr phosphatases | Reversible |
| Microcystin LR | 101043-37-2 | 995.17 | 500 nM | Acid and PP1 and PP2A | Reversible |
Handling Instruction
- Compatible with Western Blot analysis, IP, Co-IP, pull-down, IF, IHC, kinase assay and etc.
- Add concentrated cocktail at 1:100 (v/v) dilution to samples solution (such as cell lysates or tissue extracts) before assaying.
- Briefly vortex cocktails to help facilitate the dissolution.
Features
- Accurate Ingredients —— Clearance of components and concentrations to avoid experimental uncertainty
- Comprehensive Protection—— Effectively inhibit various proteases to protect proteins from being degraded
- Cost-Effective —— Lower with higher quality
Notice
- Following initial thaw, aliquot and freeze (-20°C) to avoid thawing and refreezing repeatedly.
- If Cocktail I and Cocktail II are used together, do not mix tube I with tube II beforehand, as there may be precipitation. To avoid this, please add them step by step during experiment.
Instruction Manual
- C0003-Instruction-Manual(1.51 MB)
Keywords
Phosphatase Inhibitor Cocktail II (100× DMSO)