PROM1 (AC133, Antigen AC133, CD133, CORD12, Hematopoietic Stem Cell Antigen, HProminin, MCDR2, MSTP061, PROM 1, PROM-1, Prominin-1, Prominin 1, Prominin1, Prominin-like Protein 1, PROML1, RP41, STGD4)

Prominin-1, also known as CD133, is a glycoprotein that in humans is encoded by the PROM1 gene. It is mapped to 4p15.32. Prominin-1 is a member of pentaspan transmembrane glycoproteins (5-transmembrane, 5-TM), which specifically localize to cellular protrusions. This gene encodes a pentaspan transmembrane glycoprotein. The protein localizes to membrane protrusions and is often expressed on adult stem cells, where it is thought to function in maintaining stem cell properties by suppressing differentiation. It has been proposed to act as an organizer of cell membrane topology. Prominin-1 was expressed not only on metastatic colon cancer cells, but also on differentiated colonic epithelium in both adult mice and humans.||Applications: |Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested.||Recommended Dilutions:|Western Blot: 0.1-0.5ug/ml, the detection limit is ~0.25ng/lane under reducing conditions.|Immunohistochemistry (FFPE): 0.5-1ug/ml. Boiling the paraffin sections in 10mM citrate buffer, pH 6.0, for 20 minutes is required for the staining of formalin/paraffin sections.|Optimal dilutions to be determined by the researcher.||Storage and Stability:|Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.