Protease, Serine 8 (PRSS8) Recombinant, Rat

Referencia 156483-10ug

embalaje : 10ug

Marca : US Biological

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156483 Protease, Serine 8 (PRSS8) Recombinant, Rat

Clone Type
Polyclonal
Swiss Prot
Q9ES87
Grade
Highly Purified
Shipping Temp
Blue Ice
Storage Temp
-70°C/-20ºC

Recombinant protein corresponding to aa124-342 with two N-Terminal Tags, His-Tag and GST Tag from rat PRSS8, expressed in E. coli (Q9ES87).||Amino Acid Sequence:|SSYREEG SQGDIALIRL SSPVTFSRYI RPICLPAANA SFPNGLHCTV TGWGHVAPSV SLQTPRPLQQ LEVPLISRET CSCLYNINAV PEEPHTIQQD MLCAGYVKGG KDACQGDSGG PLSCPIDGLW YLAGIVSWGD ACGAPNRPGV YTLTSTYASW IHHHVAELQP RVVPQTQESQ PDGHLCNHHP VFNLAAAQKL SRPILFLPLS LTLGLFSLWL EH||Predicted Molecular Weight:|53.8kD||Predicted isoelectric point:|6.2||Subcellular Location: |Cell membrane; Single-pass membrane protein||Applications:|Suitable for use in ELISA, Western Blot, Immunoprecipitation and SDS-PAGE.|Other applications not tested.||Recommended Dilutions:|Optimal dilutions to be determined by the researcher.||Included Protein Molecular Weight Marker: |168631: Unstained Protein Molecular Weight Marker, 10-70kD|Suppled as a liquid in 62.5mM Tris-H3PO4, pH 7.5, 1mM EDTA, 2% SDS, 100mM DTT, 1mM sodium azide, 0.01% bromo-phenol blue, 33% glycerol. Ready to use; no need to heat, dilute or add reducing agents before use.||Protein Bands: 10kD, 14kD, 18kD, 22kD, 26kD, 33kD, 44kD and 70kD.|Double Intesity Bands: The 26kD, 18kD and 10kD bands are at double intensity to make location and size approximation of proteins easier.||Usage: Allow the marker to reach room temperature and mix thoroughly before use to ensure that any solids that may have precipitated at -20°C will go back into solution. Do not boil! Load the following volumes on SDS-PAGE gel:|Mini gels: 3-5ul/well|Large gel: 7ul/well|The loading volumes listed above are recommended for gels with thickness of 0.75-1mm. The loading volume should be doubled for 1.5mm thick gels.|Store at -20°C Stable for 6 months after receipt. ||Storage and Stability:|Lyophilized powder may be stored at -70°C. Stable for 6 months after receipt. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.||Note:|Thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation by incubating the protein at 37°C for 48h, with no obvious degradation or precipitation observed. Protein loss is <5% within the expiration date under appropriate storage conditions.

Applications
Source: E. coli|Purity: ≥95%; Endotoxin: ≤1EU/ug|Form: Supplied as lyophilized powder from TRIS, 150mM sodium chloride, pH 8.0 1mM EDTA, 1mM DTT, 5% trehalose, 0.01% sarcosyl, 0.02% sodium azide. Reconstitute with sterile ddH2O.||Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Form
Supplied as lyophilized powder from PBS, pH 7.4, 1mM DTT, 5% trehalose, 0.01% sarcosyl, 0.05% Proclin-300. Reconstitute with sterile PBS to a concentration of 0.1-1mg/ml. Do not vortex.
Purity
≥95%; Endotoxin: ≤1EU/ug