Pyruvic Acid Colorimetric Assay Kit
Referencia E-BC-K130-S-100
embalaje : 100Assays
Marca : Elabscience
Contact local distributor :
Teléfono : +1 850 650 7790
| Detection Principle | Pyruvic acid can react with chromogenic agent and the reaction product is reddish brown in alkaline solution. The depth of color is directly proportional to the pyruvate content. The pyruvate content can be calculated by measuring the OD value at 505 nm. |
| Sample Type | Serum,Plasma,Tissue |
| Detection Method | Colorimetric method |
| Detection Instrument | Spectrophotometer (505 nm) |
| Research Area | Amino Acids And Proteins , Mitochondria Function , Tricarboxylic Acid（TCA）Cycle , Cuproptosis , Energy Metabolism |
| Other Reagents Required | Normal saline (0.9% NaCl), PBS (0.01 mol/L, pH 7.4) |
| Storage | This product can be stored at 2-8°C for 12 months with shading light. |
| Valid Period | 12 months |
| Sensitivity | 0.006 μmol/mL |
| Detection Range | 0.006-2.0 μmol/mL |
| Precision | inter-assay CV: 1.5% | intra-assay CV: 1.3% |
| Assay Time | 1 h |
The recommended dilution factor for different samples is as follows (for reference only):
| Sample Type | Dilution Factor |
|---|---|
| Human serum | 1 |
| Mouse serum | 1 |
| Mouse plasma | 1 |
| 10% Mouse liver tissue homogenization | 1 |
| 10% Rat kidney tissue homogenization | 1 |
| 10% Rat heart tissue homogenization | 1 |
The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.
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