- Product Name
- ACTN1 antibody
- Catalogue No.
- FNab00322
- Size
- 100μg
- Form
- liquid
- Purification
- Immunogen affinity purified
- Purity
- ≥95% as determined by SDS-PAGE
- Clonality
- polyclonal
- Isotype
- IgG
- Storage
- PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months (Avoid repeated freeze / thaw cycles.)
Immunogen
- Immunogen
- actinin, alpha 1
- Alternative Names
- Alpha-actinin-1|Alpha-actinin cytoskeletal isoform|F-actin cross-linking protein|Non-muscle alpha-actinin-1|ACTN1 antibody
- UniProt ID
- P12814
- Observed MW
- 110 kDa
Application
- Tested Applications
- ELISA, WB, IHC
- Recommended dilution
- WB: 1:500 - 1:2000; IHC: 1:50 - 1:200
Validated Images
NIH/3T3 cells were subjected to SDS PAGE followed by western blot with FNab00322( a-Actinin Antibody) at dilution of 1:1000 
Immunohistochemistry of paraffin-embedded human heart tissue slide using FNab00322( a-Actinin Antibody) at dilution of 1:200 - Background
- Alpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments. This gene encodes a nonmuscle, cytoskeletal, alpha actinin isoform and maps to the same site as the structurally similar erythroid beta spectrin gene. Three transcript variants encoding different isoforms have been found for this gene.