Rabbit Polyclonal Anti-LDL Receptor

Referencia 61099

embalaje : 0.25mL

Marca : Progen

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anti-LDL Receptor rabbit polyclonal, purified

Quantity 250 µl (100 µg/ml)
Antibody Type Polyclonal
Host Rabbit
Conjugate Unconjugated
Application IHC, Receptor binding assay, WB
Purification Affinity chromatography
Reactivity Bovine, Human, Mouse, Rat
Storage Short term at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/thaw cycles
Intended use Research use only
Immunogen Specific synthetic peptide (sequence not conserved in VLDL receptor and LRP) of the human LDL receptor extracellular domain (epitope between residues nos. 184-195, the linker region between repeats 4 and 5)
Concentration 100 µg/ml
Formulation PBS with 0.5% BSA
Note Centrifuge prior to opening
Tested applications Tested dilutions
Immunohistochemistry (IHC) - frozen 1:20-1:50
Immunohistochemistry (IHC) - paraffin 1:20-1:50 (microwave treatment recommended)
Receptor Binding Assays Assay dependent
Western Blot (WB) 1:100-1:200

The LDL receptor (160 kDa mature or glycosylated receptor; 120 kDa precursor or unglycosylated receptor) plays a key role in cellular cholesterol homeostasis.
The antibody to LDLR reacts specifically with human LDLR and is suitable for use in flow cytometry and immunoblotting. The antibody does not inhibit binding of LDL.
In immunoblotting, the antibody recognizes the 160 kDa band of LDLR and a 120 kDa band of the LDLR precursor from fibroblasts, hepatocytes, and monocytic cells cultured in the presence of lipoprotein-deficient serum.