Rat Anti-Mouse IL-2-AF488
Referencia 10202-30
embalaje : 0.1mg
Marca : Southern Biotech
Clone | JES6-5H4 |
---|---|
Isotype | Rat IgG2bκ |
Isotype Control | Rat IgG2b-AF488 (KLH/G2b-1-2) |
Specificity | Mouse IL-2 |
Alternative Names | Interleukin-2, thymocyte differentiation factor, TDF, T cell growth factor, TCGF, killer cell helper factor, KHF, macrophage-activating factor for cytotoxicity I, MAF-C I, eosinophil differentiation factor, EDF |
Immunogen | E. coli-expressed mouse IL-2 |
Conjugate | AF488 (Alexa Fluor® 488) |
Buffer Formulation | Phosphate buffered saline containing < 0.1% sodium azide |
Clonality | Monoclonal |
Concentration | 0.5 mg/mL |
Volume | 0.2 mL |
Recommended Storage | 2-8°C; Avoid exposure to light |
Trademark Information | Alexa Fluor® is a registered trademark of Thermo Fisher Scientific, Inc. or its subsidiaries |
Applications | ELISA-Detection – Quality tested 1-5,7 ELISA-Capture – Quality tested Flow Cytometry – Quality tested 8,14-17 ELISpot-Detection – Reported in literature 5,6,8-13 Immunohistochemsitry-Frozen Sections – Reported in literature 18-20 Immunoprecipitation – Reported in literature 1,2 Neutralization – Reported in literature 1,2 Multiplex-Detection – Reported in literature 3 |
RRID Number | AB_2794217 |
Gene ID | 16183 (Human) |
Gene ID Symbol | Il2 (Human) |
Gene ID Aliases | Il-2 |
UniProt ID | P04351 (Mouse |
UniProt Name | IL2_MOUSE (Mouse) |
Documentation
Technical Bulletin Safety Datasheet
Certificate of Analysis Lookup
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- 2. Abrams JS, Roncarolo M, Yssel H, Andersson U, Gleich GJ, Silver JE. Strategies of anti-cytokine monoclonal antibody development: immunoassay of IL-10 and IL-5 in clinical samples. Immunol Rev. 1992;127:5-24. (ELISA-Detection, IP, Neut)
- 3. Carson RT, Vignali DA. Simultaneous quantitation of 15 cytokines using a multiplexed flow cytometric assay. J Immunol Methods. 1999;227:41-52. (ELISA-Detection, Multiplex-Detection)
- 4. Abrams JS. Immunoenzymetric assay of mouse and human cytokines using NIP-ed anti-cytokine antibodies. Curr Protoc Immunol. 2001;6.20:1-15. (ELISA-Detection)
- 5. Pack CD, Cestra AE, Min B, Legge KL, Li L, Caprio-Young JC, et al. Neonatal exposure to antigen primes the immune system to develop responses in various lymphoid organs and promotes bystander regulation of diverse T cell specificities. J Immunol. 2001;167:4187-95. (ELISA-Detection, ELISPOT-Detection)
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- 7. Gessner A, Mohrs K, Mohrs M. Mast cells, basophils, and eosinophils acquire constitutive IL-4 and IL-13 transcripts during lineage differentiation that are sufficient for rapid cytokine production. J Immunol. 2005;174:1063-72. (ELISA-Detection)
- 8. Karulin AY, Hesse MD, Tary-Lehmann M, Lehmann PV. Single-cytokine-producing CD4 memory cells predominate in type 1 and type 2 immunity. J Immunol. 2000;164:1862-72. (ELISPOT-Detection, FC)
- 9. Stern BV, Boehm BO, Tary-Lehmann M. Vaccination with tumor peptide in CpG adjuvant protects via IFN-γ-dependent CD4 cell immunity. J Immunol. 2002;168:6099-105. (ELISPOT-Detection)
- 10. Fedoseyeva EV, Kishimoto K, Rolls HK, Illigens BM, Dong VM, Valujskikh A, et al. Modulation of tissue-specific immune response to cardiac myosin can prolong survival of allogeneic heart transplants. J Immunol. 2002;169:1168-74. (ELISPOT-Detection)
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- 12. Hofstetter HH, Mössner R, Lesch KP, Linker RA, Toyka KV, Gold R. Absence of reuptake of serotonin influences susceptibility to clinical autoimmune disease and neuroantigen-specific interferon-gamma production in mouse EAE. Clin Exp Immunol. 2005;142:39-44. (ELISPOT-Detection)
- 13. Klinman D. ELISPOT assay to detect cytokine-secreting murine and human cells. Curr Protoc Immunol. 2008;6.19:1-9. (ELISPOT-Detection)
- 14. Groux H, O'Garra A, Bigler M, Rouleau M, Antonenko S, de Vries JE, et al. A CD4+ T-cell subset inhibits antigen-specific T-cell responses and prevents colitis. Nature. 1997;389:737-42. (FC)
- 15. Tripp RA, Moore D, Jones L, Sullender W, Winter J, Anderson LJ. Respiratory syncytial virus G and/or SH protein alters Th1 cytokines, natural killer cells, and neutrophils responding to pulmonary infection in BALB/c mice. J Virol. 1999;73:7099-107. (FC)
- 16. Hamada H, Garcia-Hernandez Mde L, Reome JB, Misra SK, Strutt TM, McKinstry KK, et al. Tc17, a unique subset of CD8 T cells that can protect against lethal influenza challenge. J Immunol. 2009;182:3469-81. (FC)
- 17. Nakamura T, Nakao T, Yoshimura N, Ashihara E. Rapamycin prolongs cardiac allograft survival in a mouse model by inducing myeloid-derived suppressor cells. Am J Transplant. 2015 May 5. doi: 10.1111/ajt.13276. [Epub ahead of print]. (FC)
- 18. Hersmann GH, Kriegsmann J, Simon J, Hüttich C, Bräuer R. Expression of cell adhesion molecules and cytokines in murine antigen-induced arthritis. Cell Adhes Commun. 1998;6:69-82. (IHC-FS)
- 19. Yamagami S, Tsuru T. Increase in orthotopic murine corneal transplantation rejection rate with anterior synechiae. Invest Ophthalmol Vis Sci. 1999;40:2422-6. (IHC-FS)
- 20. Cardona AE, Restrepo BI, Jaramillo JM, Teale JM. Development of an animal model for neurocysticercosis: immune response in the central nervous system is characterized by a predominance of γδ T cells. J Immunol. 1999;162:995-1002. (IHC-FS)