Rat monoclonal antibody PE/Cy5.5 labled to CD16/CD32
Referencia CFH8954-50
embalaje : 50Assays
Marca : Cohesion Biosciences
Product Name | Anti-CD16/CD32 Antibody-PE/Cy5.5 labled |
Cat No: | CFH8954 |
Source: | Rat |
Reactivity: | M |
Applications: | IF, FC |
*Application Key: | |
E- ELISA, WB - Western blot, IH - Immunohistochemistry, IF - Immunofluorescence, FC - Flow cytometry, IC - Immunocytochemistry, IP - Immunoprecipitation, ChIP - Chromatin Immunoprecipitation, EMSA - Electrophoretic Mobility Shift Assay, BL - Blocking, SE - Sandwich ELISA, CBE - Cell-based ELISA, RNAi - RNA interference | |
*Species Reactivity Key: | |
H - Human, M - Mouse, R - Rat, B - Bovine, C - Chicken, D - Dog, G - Goat, Mk - Monkey, P - Pig, Rb - Rabbit, S - Sheep, Z - Zebrafish |
Description: Rat monoclonal antibody PE/Cy5.5 labled to CD16/CD32 Immunogen: Sorted pre-B cells Purification: The antibody was purified by affinity chromatography. Clonality: Monoclonal (clone: 93) Conjugation: PE/Cy5.5 Form: Rat IgG2a lambda. Liquid in PBS, pH 7.3, and 0.02% sodium azide. Dilution: 10 μl / assay Gene Symbol: FCGR2B; FCGR3A Alternative Names: CD32; FCG2; IGFR2; Low affinity immunoglobulin gamma Fc region receptor II-b; IgG Fc receptor II-b; CDw32; Fc-gamma RII-b; Fc-gamma-RIIb; FcRII-b; CD32Entrez Gene (Mouse) : SwissProt (Mouse) : Directions for Use : 1. Take 100 μl peripheral blood anticoagulated by EDTA and add to the bottom of 5 ml tube. 2. Add 10 μl labeled antibody to the bottom of flow tube mixing with the whole blood, incubate for 20 minutes at room temperature away from light. 3. Add 2 ml RBC lysis buffer, incubate for 10 minutes away from light after mixing, dissolve red blood cells. 4. Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant. 5. Add 2 ml PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant. 6. Add 0.5 ml PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured).Storage/Stability : Shipped and store at 4°C for one year. Do not freeze.