RD growing culture
Referencia 330401
embalaje : 1cellcultureflask
Marca : CLS Cell Lines Service
RD Cells
General information
Description | This line has recently been shown to be at least parental, if not identical, to TE-671. |
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Organism | Human |
Tissue | Embryonic |
Disease | Rhabdomyosarcoma |
Synonyms | R D, RD-2, RD 2, 130T, 130-T, 130 T, TE-32, TE 32, TE32, TE 32.T, Te 32.T |
Characteristics
Age | Embryo |
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Gender | Female |
Ethnicity | Caucasian |
Cell type | Spindle cells and large multinucleated cells |
Growth properties | Adherent |
Identifiers / Biosafety / Citation
Citation | RD (Cytion catalog number 300401) |
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Biosafety level | 1 |
Expression / Mutation
Isoenzymes | G6PD, B |
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Virus susceptibility | Poliovirus 1, vesicular stomatitis (Indiana), herpes simplex, vaccinia |
Reverse transcriptase | Negative |
Products | Myoglobin, myosin ATPase |
Karyotype | 2n=48 |
Handling
Culture Medium | DMEM, w: 4.5 g/L Glucose, w: 4 mM L-Glutamine, w: 1.5 g/L NaHCO3, w: 1.0 mM Sodium pyruvate (Cytion article number 820300a) |
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Medium supplements | Supplement the medium with 10% FBS |
Passaging solution | Accutase |
Subculturing | Remove the old medium from the adherent cells and wash them with PBS that lacks calcium and magnesium. For T25 flasks, use 3-5 ml of PBS, and for T75 flasks, use 5-10 ml. Then, cover the cells completely with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. Let the cells incubate at room temperature for 8-10 minutes to detach them. After incubation, gently mix the cells with 10 ml of medium to resuspend them, then centrifuge at 300xg for 3 minutes. Discard the supernatant, resuspend the cells in fresh medium, and transfer them into new flasks that already contain fresh medium. |
Split ratio | A ratio of 1:2 is recommended |
Fluid renewal | Every 3 to 4 days |
Freeze medium | CM-1 (Cytion catalog number 800100) or CM-ACF (Cytion catalog number 806100) |
Handling of cryopreserved cultures |
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Quality control / Genetic profile / HLA
Sterility | Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections. |
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STR profile | Amelogenin: x,x CSF1PO: 10,11 D13S317: 13 D16S539: 10,11 D5S818: 11 D7S820: 8,12 TH01: 9.3 TPOX: 9 vWA: 18 D3S1358: 15,17 D21S11: 28,29 D18S51: 13,18 Penta E: 12 Penta D: 11,13 D8S1179: 11,15 FGA: 20,21 |
HLA alleles | A*: 01:01:01 B*: 37:01:01 C*: 06:02:01 DRB1*: 03:01:01 DQA1*: 05:01:01 DQB1*: 02:01:01 DPB1*: 01:01:01 E: 01:01:01 |