Recombinant Human Cuteaneous T-cell Attracting Chemokine/CCL27（E.coli ）

Biological Activity

Genus: Human
Expression system: E.coli
Purity: >96% by SDS-PAGE and HPLC analysis
Endotoxin: rHuCTACK/CCL27 is less than 0.1EU/µg as determined by LAL method
Molecular weight: about 10.1 kDa, a non-glycosylated polypeptide chain containing 88 amino acids
Storage and stability: Use a manually defrosted refrigerator and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C, supplied as is. 1 month, reconstituted at 2 to 8 °C under sterile conditions. 3 months, reconstituted at -20 to -70 °C under sterile conditions.


Bioactivity: Fully bioactive compared to standard. Bioactivity was determined by chemotaxis bioassay using CCR10 transfected BaF3 cells in the concentration range of 10-100 ng/ml.

Chemical Information

Solubility (25°C)	Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL.
Storage	Powder          -20°C   3 years ;  4°C   2 years In solvent       -80°C   6 months ;  -20°C   1 month

References

[1] Shanmugapriya Thangavadivel, et al. Oncotarget. CCR10/CCL27 crosstalk contributes to failure of proteasome-inhibitors in multiple myeloma

[2] Lenie J van den Broek, et al. Tissue Eng Part A. Differential response of human adipose tissue-derived mesenchymal stem cells, dermal fibroblasts, and keratinocytes to burn wound exudates: potential role of skin-specific chemokine CCL27

[3] G Goteri, et al. Br J Dermatol. Serum and tissue CTACK/CCL27 chemokine levels in early mycosis fungoides may be correlated with disease-free survival following treatment with interferon alfa and psoralen plus ultraviolet A therapy

[4] Kimberly A Kraynyak, et al. Vaccine. Systemic immunization with CCL27/CTACK modulates immune responses at mucosal sites in mice and macaques

[5] Takako Hirata, et al. J Biol Chem. Human P-selectin glycoprotein ligand-1 (PSGL-1) interacts with the skin-associated chemokine CCL27 via sulfated tyrosines at the PSGL-1 amino terminus