Recombinant Human TNF-β (E. coli）

Biological Activity

Genus: Human
Expression system: E. coli
Endotoxin: < 0.2 EU/μg of protein by gel coagulation
Purity: >95% by SDS-PAGE analysis
Apparent molecular weight: ~18.6 kDa on SDS-PAGE under reducing conditions
Storage and Stability: This product is stable for up to 6 months at temperatures below -70°C after receipt. After reconstitution, the product should be stable for 1 week at 4°C or for 3 months at -20°C. For long-term storage, the addition of a carrier protein (e.g. 0.1% BSA) is recommended. Avoid repeated freeze-thaw cycles.


Bioactivity: TNF is secreted by macrophages, monocytes, neutrophils, T cells, and NK cells upon stimulation by bacterial LPS. Cells expressing CD4 secrete TNF-α, while CD8 cells secrete little or no TNF-α. TNF-α synthesis is induced by many different stimuli, including interferon, IL2, and GM-CSF. TNF-β is a potent mediator of inflammatory and immune responses. It belongs to the TNF family of ligands and signals through TNFR1 and TNFR2. TNF-β is produced by activated T and B lymphocytes and has similar activity to TNF-α. It mediates a large number of inflammatory, immunostimulatory and antiviral responses.

Chemical Information

Solubility (25°C)	Reconstitute the lyophilized powder in ddH₂O or PBS up to 100 μg/ml.
Storage	Powder          -20°C   3 years ;  4°C   2 years In solvent       -80°C   6 months ;  -20°C   1 month

References

[1] Carina C Dos Santos, et al. Vaccines (Basel). Recombinant BCG-LTAK63 Vaccine Candidate for Tuberculosis Induces an Inflammatory Profile in Human Macrophages

[2] Zhenling Ma, et al. Protein J. Expression and purification of recombinant human CCL5 and its biological characterization

[3] Hossein Safarpour, et al. Int J Biol Macromol. Expression analysis and ATR-FTIR characterization of the secondary structure of recombinant human TNF-α from Escherichia coli SHuffle® T7 Express and BL21 (DE3) cells

[4] Andreas Barratt-Due, et al. Mol Immunol. Selective inhibition of TNF-alpha or IL-1 beta does not affect E. coli-induced inflammation in human whole blood

[5] B B Aggarwal, et al. Ciba Found Symp. Human tumour necrosis factors: structure and receptor interactions