Recombinant Human TNF RII (Mammalian, C-6His)

Biological Activity

Recombinant Human TNF RII belongs to the TNFR (tumor necrosis factor receptor) superfamily characterized by cysteine-rich extracellular domains. TNFRII is strongly expressed at the cartilage-pannus junction, and plays a major role in a subset of families with multiple cases of rheumatoid arthritis (RA). This receptor mediates most of the metabolic effects of TNF-alpha function by antagonizing its biological activity.

Recombinant Human Tumor Necrosis Factor Receptor II expressed the target gene encoding Leu23-Asp257 with a 6His tag at the C-terminus.

The ED50 of Recombinant Human TNF RII (C-6His) is 0.212 ug/ml.

Accession: P20333

Endotoxin < 1 EU/µg

Apparent Molecular Weight: 40 KDa, reducing conditions

Lyophilized from a 0.2 μm filtered solution of 20mM PB, 150mM NaCl, pH 7.4.

Chemical Information

Form	Lyophilized powder
Solubility (25°C)	Reconstitute the lyophilized powder in distilled water up to 100 μg/ml.
Storage	Stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days and at -20°C for 3 months.

References

[1] Dao Xuan Nguyen, et al. J Exp Med. Anti-TNF drives regulatory T cell expansion by paradoxically promoting membrane TNF-TNF-RII binding in rheumatoid arthritis

[2] M Aringer, et al. Lupus. Increased bioactive TNF in human systemic lupus erythematosus: associations with cell death

[3] C K Edwards 3rd. Ann Rheum Dis. PEGylated recombinant human soluble tumour necrosis factor receptor type I (r-Hu-sTNF-RI): novel high affinity TNF receptor designed for chronic inflammatory diseases

[4] M Aoki, et al. J Vet Med Sci. Cytotoxicity induced by recombinant human tumor necrosis factor-alpha dependent on the types of its receptors on canine cells

[5] A Gessl, et al. Immunology. Tumour necrosis factor-alpha augments the expression of Fc IgE receptor (Fc epsilon RII/CD23) on human monocytic cell lines and down-regulates interleukin-4-driven Fc epsilon RII expression on monocytes