Recombinant Protein Human IL-3

Symansis' purified IL-3^hcx has N-linked and possibly O-linked oligosaccharides, shown by a drop in observed MW on SDS-PAGE after treatment with PNGase F and a possible further drop in observed MW on SDS-PAGE after treatment with a glycosidase cocktail. Symansis' IL-3^hcx consists of 0-40% carbohydrate by weight.

Densitometry of protein isoforms visualised by 2-DE. Spot trains are normal in human proteins and highlight the presence of multiple isoforms which differ according to their level of post-translational modification. The triangle indicates the theoretical MW and pI of the protein.

1. Lutz MB (2004) Immunobiology 209: 79-87.
2. Ding H, et al. (2003) Protein Expr Purif. 31: 34-41.