Recombinant Rat GM-CSF Protein (CHO)

Biological Activity

Granulocyte Macrophage-Colony Stimulating Factor (GM-CSF) is produced by a number of different cell types, including activated T cells, B cells, macrophages, mast cells, endothelial cells, and fibroblasts, in response to cytokine of immune and inflammatory stimuli. GM-CSF was initially characterized as a growth factor that can support the in vitro colony formation of granulocyte-macrophage progenitors. Besides granulocyte-macrophage progenitors, GM-CSF is a growth factor for erythroid, megakaryocyte, and eosinophil progenitors. Additionally, Granulocyte Macrophage-Colony Stimulating Factor (GM-CSF) can stimulate the proliferation of a number of tumor cell lines, including osteogenic sarcoma, carcinoma, and adenocarcinoma cell lines.

ED50 < 5.0 pg/ml, measured in a cell proliferation assay using FDC-P1 cells, corresponding to a specific activity of > 2.0 × 10⁸ units/mg.

Apparent Molecular Weight: 16~26 kDa, on SDS-PAGE under non-reducing conditions.

Accession # P48750-1

Upon receiving, this product remains stable for up to 6 months at lower than -70°C. Upon reconstitution, the product should be stable for up to 1 week at 4°C or up to 3 months at -20°C. For long term storage it is recommended that a carrier protein (example 0.1% BSA) be added. Avoid repeated freeze-thaw cycles.

Chemical Information

Solubility (25°C)	Reconstitute the lyophilized powder in ddH₂O or PBS up to 100 μg/ml
Storage	Powder          -20°C   3 years ;  4°C   2 years In solvent       -80°C   6 months ;  -20°C   1 month

References

[1] Pravin Yeapuri, et al. J Control Release. Development of an extended half-life GM-CSF fusion protein for Parkinson's disease

[2] Mara de Los Milagros Sales, et al. J Biotechnol. Bifunctional GM-CSF-derived peptides as tools for O-glycoengineering and protein tagging

[3] Ming Li, et al. Tumour Biol. A novel recombinant protein of ephrinA1-PE38/GM-CSF activate dendritic cells vaccine in rats with glioma

[4] C Wiltschke, et al. Immunobiology. Murine recombinant GM-CSF-driven rat bone marrow cell differentiation and factors suppressing cell proliferation

[5] M S Cairo. Am J Pediatr Hematol Oncol. Review of G-CSF and GM-CSF. Effects on neonatal neutrophil kinetics