- More Files
Western Blot
Western blot analysis of His-tag polyclonal antibody (1:500) and Goat Anti-Rabbit IgG (H&L) secondary antibody (HRP) (1:20000), Lane 1: 293F cell lysate, Lane 2: 293F cell transfection pcDNA3.0-ORF6-His. Western blot analysis of SARS-CoV-2 ORF6 polyclonal antibody (1:500) and Goat Anti-Rabbit IgG (H&L) secondary antibody (HRP) (1:20000), Lane 3: 293F cell transfection pcDNA3.0-ORF6-His, Lane 4: 293F cell lysate (negative control).
- Specifications
Product Description
Rabbit polyclonal antibody raised against partial synthetic peptide of SARS-CoV-2 ORF6.
Immunogen
A synthetic peptide corresponding to amino acids 21-61 of SARS-CoV-2 ORF6.
Host
Rabbit
Reactivity
SARS-CoV-2
Specificity
The antibody detects endogenous levels of SARS-CoV-2 ORF6.
Form
Liquid
Purification
Affinity purification
Purity
> 95% by SDS-PAGE
Isotype
IgG
Recommend Usage
ELISA
Western Blot (1:500)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.2 (0.02% sodium azide).
Storage Instruction
Store at 4°C for short term. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Applications
Western Blot
Western blot analysis of His-tag polyclonal antibody (1:500) and Goat Anti-Rabbit IgG (H&L) secondary antibody (HRP) (1:20000), Lane 1: 293F cell lysate, Lane 2: 293F cell transfection pcDNA3.0-ORF6-His. Western blot analysis of SARS-CoV-2 ORF6 polyclonal antibody (1:500) and Goat Anti-Rabbit IgG (H&L) secondary antibody (HRP) (1:20000), Lane 3: 293F cell transfection pcDNA3.0-ORF6-His, Lane 4: 293F cell lysate (negative control).Enzyme-linked Immunoabsorbent Assay
- Publication Reference
- Evolution of enhanced innate immune suppression by SARS-CoV-2 Omicron subvariants.
Ann-Kathrin Reuschl, Lucy G Thorne, Matthew V X Whelan, Roberta Ragazzini, Wilhelm Furnon, Vanessa M Cowton, Giuditta De Lorenzo, Dejan Mesner, Jane L E Turner, Giulia Dowgier, Nathasha Bogoda, Paola Bonfanti, Massimo Palmarini, Arvind H Patel, Clare Jolly, Greg J Towers.
Nature Microbiology 2024 Feb; 9(2):451.
Application：WB, Human, Calu-3 cell line.
- Nanoscopic Elucidation of Spontaneous Self-Assembly of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) Open Reading Frame 6 (ORF6) Protein.
Goro Nishide, Keesiang Lim, Maiki Tamura, Akiko Kobayashi, Qingci Zhao, Masaharu Hazawa, Toshio Ando, Noritaka Nishida, Richard W Wong.
The Journal of Physical Chemistry Letters 2023 Sep; 14(38):8385.
Application：SDS-PAGE, WB, Human, A549, PC-9 cells, Recombinant proteins.
- Sarbecovirus ORF6 proteins hamper induction of interferon signaling.
Izumi Kimura, Yoriyuki Konno, Keiya Uriu, Kristina Hopfensperger, Daniel Sauter, So Nakagawa, Kei Sato.
Cell Reports 2021 Mar; 34(13):108916.
Application：WB-Tr, Human, HEK 293 cells.
- Evolution of enhanced innate immune suppression by SARS-CoV-2 Omicron subvariants.