STREPTAVIDIN PEROXIDASE Conjugated
Referencia S000-03
embalaje : 1mg
Marca : Rockland Immunochemicals
Contact local distributor :
Teléfono : +1 850 650 7790
Specifications for Streptavidin Peroxidase Conjugated
Product Details
Streptavidin Peroxidase Conjugated - S000-03
HRP-SA, Horseradish Peroxidase conjugated S avidin, Streptavidin HRP, Streptavidin conjugated to horseradish peroxidase (HRP), HRP-linked Streptavidin
Peroxidase (HRP)
Target Details
Streptavidin-HRP was prepared from chromatographically purified streptavidin. Streptavidin Peroxidase conjugate was assayed by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase and anti-Streptavidin.
P22629 - UniProtKB
CAA00084.1 - NCBI Protein
Application Details
Dot Blot, WB
ELISA, EM, IF, IHC, Other - View References
Streptavidin Peroxidase has been tested by dot blot and western blot and is a useful detection reagent for primary antibodies conjugated to biotin. Streptavidin Peroxidase can be utilized in Immunohistochemistry, Immunofluorescence, immuno-EM, Western Blotting, and ELISA experiment formats in combination with the proper substrate (TMB-1000 or FEMTOMAX-110).
Formulation
1.0 mg/mL by UV absorbance at 280 nm
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!
10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
1.0 mL
Restore with deionized water (or equivalent)
Shipping & Handling
Ambient
Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. Streptavidin Peroxidase conjugated is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Expiration date is one (1) year from date of receipt.
Background
Streptavidin is isolated from bacteria, Streptomyces avidinii, and has an exceptionally high binding affinity for B7 (biotin). Rockland offers streptavidin in unconjugated and conjugated forms for common immunoassays including ELISA, western blotting, immunohistochemistry. Streptavidin is a tetrameric protein capable of binding 4 biotin groups to each molecule of streptavidin. While streptavidin has identical binding properties as avidin, it lacks the glycoprotein portion of the molecule and therefore shows less non-specific binding. Streptavidin is a slightly smaller molecule with a molecular weight of approximately 53.6 kDa. The sequence of avidin only shows 30% homology with streptavidin, and anti-avidin and anti-streptavidin antibodies are not immunologically cross reactive. Horseradish Peroxidase (HRP) is an enzyme that utilize organic peroxide compounds as electron donors. Naturally provides protection for plants against pathogens, but can be utilized in molecular biology to convert various substrates to detectable compounds (such as in Western Blotting and ELISAs).
References (37)
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Applications
IHC, ICC, Histology PubMed
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IHC, ICC, Histology PubMed
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IHC, ICC, Histology PubMed
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Applications
IHC, ICC, Histology PubMed
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Applications
IHC, ICC, Histology PubMed
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IHC, ICC, Histology PubMed
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IHC, ICC, Histology PubMed
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IHC, ICC, Histology PubMed
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Undefined PubMed
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IHC, ICC, Histology PubMed
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Applications
IHC, ICC, Histology PubMed
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Applications
Undefined PubMed
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Applications
WB, IB, PCA PubMed
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Applications
IHC, ICC, Histology PubMed
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Applications
IF, Confocal Microscopy PubMed
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Applications
WB, IB, PCA PubMed
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Applications
IHC, ICC, Histology PubMed
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IHC, ICC, Histology Source
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EIA, ELISA PubMed
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Applications
IHC, ICC, Histology PubMed
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Immuno-EM PubMed
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Immuno-EM PubMed
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E, EIA PubMed
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E, EIA PubMed
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WB, IB, PCA PubMed
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Applications
IHC, ICC, Histology PubMed
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WB, IB, PCA PubMed
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E, EIA PubMed
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Applications
IHC, ICC, Histology PubMed
Related Protocols
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Heat-induced Antigen Retrieval Protocol
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Immunofluorescence (IF) Protocol
Immunohistochemistry (IHC) Protocol
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IP-WB with TrueBlot® Protocol
Multi-Lysate Western Blotting Protocol
Nuclear & Cytoplasmic Extract Protocol
Protease-induced Antigen Retrieval Protocol
Sandwich ELISA Protocol for Collagen
Staining Paraffin Sections by PAP Procedure
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Certificate of Analysis Lookup
Disclaimer
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
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