Thioflavin T [2390-54-7]
Referencia HY-D0218-1ml
embalaje : 10mM/1mL
Marca : MedChemExpress
Thioflavin T is a cationic Benzothiazole dye that shows enhanced fluorescence upon binding to amyloid in tissue sections.
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Thioflavin T Estructura química
No. CAS : 2390-54-7
This product is a controlled substance and not for sale in your territory.
Based on 10 publication(s) in Google Scholar
Descripciòn |
Thioflavin T is a cationic Benzothiazole dye that shows enhanced fluorescence upon binding to amyloid in tissue sections. |
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In Vitro |
Thioflavin T (ThT) is a benzothiazole dye that exhibits enhanced fluorescence upon binding to amyloid fibrils and is commonly used to diagnose amyloid fibrils, both ex vivo and in vitro. In aqueous solutions, Thioflavin T is found to exist as micelles at concentrations commonly used to monitor fibrils by fluorescence assay (~10-20 μM). Specific conductivity changes are measured at varying concentration of Thioflavin T and the critical micellar concentration is calculated to be 4.0±0.5 μM. Changes in the fluorescence excitation and emission of Thioflavin T are also dependent on the micelle formation. The Thioflavin T micelles of 3 nm diameter are directly visualized using atomic force microscopy, and bound Thioflavin T micelles are observed along the fibril length for representative fibrils. Increasing concentration of Thioflavin T above the critical micellar concentration shows increased numbers of micelles bound along the length of the amyloid fibrils. Thioflavin T micelles are disrupted at low pH as observed by atomic force microscopy and fluorescence enhancement upon binding of Thioflavin T to amyloid fibrils also reduced by several-fold upon decreasing the pH to below 3.The micelles of Thioflavin T bind amyloid fibrils leading to enhancement of fluorescence emission[1]. MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. |
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Peso molecular |
318.86 |
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Fòrmula |
C17H19ClN2S |
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No. CAS |
2390-54-7 |
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Unlabeled CAS |
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Appearance |
Solid |
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Color |
Yellow to orange |
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SMILES |
CC1=CC=C2[N+](C)=C(C3=CC=C(N(C)C)C=C3)SC2=C1.[Cl-] |
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Envío | Room temperature in continental US; may vary elsewhere. |
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Almacenamiento |
4°C, sealed storage, away from moisture and light *In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light) |
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Solvente y solubilidad |
In Vitro:
DMSO : 16.67 mg/mL (52.28 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO) H2O : 5 mg/mL (15.68 mM; ultrasonic and warming and heat to 60°C) Preparing
Stock Solutions
View the Complete Stock Solution Preparation Table
*
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles. * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol) Concentration (start) × Volume (start) = Concentration (final) × Volume (final) This equation is commonly abbreviated as: C1V1 = C2V2 In Vivo:
Select the appropriate dissolution method based on your experimental animal and administration route.
For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
Dosage mg/kgAnimal weight Dosing volume Number of animals Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO
+
%
+
%
Tween-80
+
%
Saline
The co-solvents required include: DMSO,
. All of co-solvents are available by MedChemExpress (MCE).
, Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration:
mg/mL
Method for preparing stock solution:
mg
drug dissolved in
μL
DMSO (Stock solution concentration: mg/mL).
*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take
μL DMSO stock solution, add
μL .
μL , mix evenly, next add
μL Tween 80, mix evenly, then add
μL Saline.
If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
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Pureza y Documentación |
Purity: 98.45% |
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Dyeing Example |
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Referencias |
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Complete Stock Solution Preparation Table
*
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
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H2O / DMSO | 1 mM | 3.1362 mL | 15.6809 mL | 31.3617 mL | 78.4043 mL |
5 mM | 0.6272 mL | 3.1362 mL | 6.2723 mL | 15.6809 mL | |
10 mM | 0.3136 mL | 1.5681 mL | 3.1362 mL | 7.8404 mL | |
15 mM | 0.2091 mL | 1.0454 mL | 2.0908 mL | 5.2270 mL | |
DMSO | 20 mM | 0.1568 mL | 0.7840 mL | 1.5681 mL | 3.9202 mL |
25 mM | 0.1254 mL | 0.6272 mL | 1.2545 mL | 3.1362 mL | |
30 mM | 0.1045 mL | 0.5227 mL | 1.0454 mL | 2.6135 mL | |
40 mM | 0.0784 mL | 0.3920 mL | 0.7840 mL | 1.9601 mL | |
50 mM | 0.0627 mL | 0.3136 mL | 0.6272 mL | 1.5681 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Thioflavin T Related Classifications
- Neuronal Signaling
- Amyloid-β
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Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.
Keywords:
Thioflavin T2390-54-7Basic Yellow 1Basic Yellow1Basic Yellow-1Amyloid-ββ-amyloid peptideAβAbetaInhibitorinhibitorinhibit