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> TLR4 (Toll Like Receptor 4, CD284) (APC)

TLR4 (Toll Like Receptor 4, CD284) (APC)

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Referencia T8050-34-APC-100ul

embalaje : 100ul

Marca : US Biological

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T8050-34-APC TLR4 (Toll Like Receptor 4, CD284) (APC)

Clone Type
Polyclonal
Host
mouse
Source
human
Isotype
IgG2a
Grade
Affinity Purified
Applications
IP
Crossreactivity
Bo Ca Gp Hu Mk Po
Shipping Temp
Blue Ice
Storage Temp
4°C Do Not Freeze

TLR4 has been demonstrated to act as a receptor for LPS on human monocytes and macrophages. TLR4 signalling of LPS stimulation requires the presence of the MD-2 molecule.|TLR4 is weakly expressed by resting cells, but is upregulated following stimulation with LPS. This antibody has been demonstrated to block activation of monocytes with LPS. Removal of sodium azide is recommended prior to use in functional assays. ||Applications:|Suitable for use in Immunoprecipitation. Other applications not tested.||Recommended Dilution:|Optimal working dilutions to be determined by researcher.||Hybridoma: |Sp2/0 myeloma cells with spleen cells from Balb/c mice. ||Storage and Stability:|Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.||Note: Applications are based on unconjugated antibody.

Applications
Product Type: Mab|Isotype: IgG2a|Clone No: 3F5 (HTA125)|Host: mouse|Source: human|Concentration: As Reported|Form: Supplied as a liquid in PBS, pH 7.2. Labeled with Allophycocyanin (APC).|Purity: Purified by Protein A affinity chromatography from tissue culture supernatant.|Immunogen: Ba/F3 cell line expressing TLR4|Specificity: Recognizes human TLR4 (Toll Like Receptor 4). Has been shown to block the activation of monocytes with LPS. Species Crossreactivity: canine, rhesus monkey, guinea pig, porcine, bovine and mouse||Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Immunogen
Ba/F3 cell line expressing TLR4
Form
Supplied as a liquid in PBS, pH 7.2. Labeled with Allophycocyanin (APC).
Purity
Purified by Protein A affinity chromatography from tissue culture supernatant.
Specificity
Recognizes human TLR4 (Toll Like Receptor 4). Has been shown to block the activation of monocytes with LPS. Species Crossreactivity: canine, rhesus monkey, guinea pig, porcine and bovine
References
1. Shimazu, R., et al., J. Exp. Med. 189: 1,777-1,782 (1999). 2. Jiang, Q., et al., J. Immunol. 165: 3,541-3,544 (2000). 3. Yang, S., et al., Infect. Immun. 69: 2,045-2,053 (2001). 4. Triantafilou, M., et al., J. Cell Sci. 115: 2,603-2,611 (2002).