TransScript® Reverse Transcriptase
Referencia AT101-03
embalaje : 5×10000units
Marca : TransGen Biotech
Contact local distributor :
Teléfono : +1 850 650 7790
ManualTransScript® Reverse Transcriptase is a recombinant M-MLV reverse transcriptase with deficient RNase H activity.
• Deficient RNase H activity to reduce RNA template degradation during the first-strand cDNA synthesis.
• Anchored Oligo(dT)18 Primer for higher yield and more full length cDNA.
• cDNA up to 12 kb.
Applications
• First-strand cDNA synthesis
• Multiple copy and low copy gene detection
Storage
at -20 ℃ for two years
Shipping
Dry ice (-70 ℃)
| Component | AT101-02 | AT101-03 |
| TransScript® RT | 10000 U | 5×10000 U |
| 5×TS RT Buffer | 200 μl | 5×200 μl |
| Anchored Oligo(dT)18 Primer (0.5 μg/μl) | 50 μl | 250 μl |
Wu L, Liu D, Wu J, et al. Regulation of FLOWERING LOCUS T by a microRNA in Brachypodium distachyon.Plant Cell[J]. 2013, 25(11):4363-77.
Meng X , Jiang Q , Chang N , et al. Small activating RNA binds to the genomic target site in a seed-region-dependent manner[J]. Nucleic Acids Research, 2016, 44(5):2274-2282.
Xiao G , Wan Z , Fan Q , et al. The metal transporter ZIP13 supplies iron into the secretory pathway in Drosophila melanogaster.[J]. Elife, 2014, 3(16):e03191.
Tang X , Zhou B . Ferritin is the key to dietary iron absorption and tissue iron detoxification in Drosophila melanogaster[J]. The FASEB Journal, 2013, 27(1):288-298.
Zheng J , Zhang L , Zhang J , et al. Single modification at position 14 of siRNA strand abolishes its gene-silencing activity by decreasing both RISC loading and target degradation[J]. The FASEB Journal, 2013, 27(10):4017-4026.