K2127-100 assays | Triglyceride Quantification Colorimetric/Fluorometric

Background

Triglycerides (TG) is an ester derived from three fatty acids and one glycerol and are the main constituent of LDL, VLDL, vegetable oil and animal fat. TG plays an important role in transporting fatty acids and acts as an energy source. TG are broken down into fatty acids and glycerol, both of which can serve as substrates for metabolic pathways and energy producing. High blood levels of TG are associated with pancreatitis, atherosclerosis, heart disease and stroke.

The Triglyceride Quantification Colorimetric/Fluorometric Kit provides a sensitive, fast and convenient way for detection of TG levels in various biological samples based on colorimetric and fluorometric method. In the assay, TG are converted to free fatty acids and glycerol, which is then oxidized to generate a product which reacts with Triglyceride Probe to yield fluorescence (Ex/Em = 535/587 nm) and color (spectrophotometry at λ = 570 nm). The assay can detect 2 pmol-10nmol (or 2-10000 µM range) of triglyceride in various biological samples. The kit can also detect monoglycerides and diglycerides.

Features & Properties

Features	Fast and convenient; Simple procedure; Takes approx. 1 hour; Kit contains the necessary reagents for accurate measurement of triglyceride in various biological samples.
Shipping	Blue Ice
Storage Conditions	Store at -20°C.

Quality Control

DataSheet

Storage

Store at -20°C.

Overview

Kit components

Triglyceride Assay Buffer, Triglyceride Probe (in DMSO, anhydrous), Lipase, Triglyceride Enzyme Mix (lyophilized), Triglyceride Standard (1 mM)

Triglyceride Quantification Colorimetric/Fluorometric Kit

Referencia K2127-100 assays

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Marca : APExBIO Technology