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> Triton X-100 [9002-93-1]

Triton X-100 [9002-93-1]

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Triton X-100 is a non-denaturing detergent that solubilizes lipid membranes. Triton X-100 is commonly used in laboratories and is applied to vaccines at different stages of the manufacturing process. Triton X-100 is listed as an excipient in certain vaccines including split virus influenza vaccines. Triton X-100 is a nonionic surfactant.

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Triton X-100 Estructura química

Triton X-100 Estructura química

No. CAS : 9002-93-1

This product is a controlled substance and not for sale in your territory.

Based on 10 publication(s) in Google Scholar

Other Forms of Triton X-100:

  • Triton X-100 surfactant Obtener un presupuesto
Descripciòn

Triton X-100 is a non-denaturing detergent that solubilizes lipid membranes. Triton X-100 is commonly used in laboratories and is applied to vaccines at different stages of the manufacturing process. Triton X-100 is listed as an excipient in certain vaccines including split virus influenza vaccines. Triton X-100 is a nonionic surfactant[1][2].

In Vitro

Triton X-100 (0.5%-0.05%, 1 h) reduces the infectivity of a classical enveloped virus such as West Nile virus (WNV)[3].
Triton X-100 (0.19-0.20 mM) makes the HeLa cells underwent irreversible permeabilization of the membrane and structural collapse[4].
Triton X-100 (0.01%, 1 h) induces apoptotic and necrotic death in prostate and colon cancer cell lines (PC-3, SW-620 and HT-29 cells), characterized by the typical morphological features and internucleosomal DNA fragmentation[5].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Triton X-100 Related Antibodies
No. CAS

9002-93-1
Appearance

Liquid (Density: 1.065 g/cm3)

Color

Colorless to light yellow

SMILES

CC(C)(C)CC(C)(C)C1=CC=C(OCCO)C=C1.12
Envío

Room temperature in continental US; may vary elsewhere.
Almacenamiento
Pure form -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvente y solubilidad
In Vitro: 

H2O : ≥ 200 mg/mL

DMSO : 100 mg/mL (ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

*"≥" means soluble, but saturation unknown.

  • Calculadora de molaridad

  • Calculadora de dilución

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.5 mg/mL; Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 2.5 mg/mL; Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
Pureza y Documentación

Purity: 99.8%

SDS (758 KB)

COA (242 KB)

Instrucciones de manejo (2659 KB)
Referencias

  • [1]. Herman SM, et al. The hidden allergen: Triton X-100, a derivative of polyethylene glycol. J Allergy Clin Immunol Pract. 2021;9(7):2941.  [Content Brief]

    [2]. Ghosh R, et al. Unraveling diverse action of triton X-100 and methimazole on lysozyme fibrillation/aggregation: Physicochemical insights. Int J Biol Macromol. 2021;167:736-745.  [Content Brief]

    [3]. Colavita F, et al. Evaluation of the inactivation effect of Triton X-100 on Ebola virus infectivity. J Clin Virol. 2017 Jan;86:27-30.  [Content Brief]

    [4]. Koley D, et al. Triton X-100 concentration effects on membrane permeability of a single HeLa cell by scanning electrochemical microscopy (SECM). Proc Natl Acad Sci U S A. 2010 Sep 28;107(39):16783-7.  [Content Brief]

    [5]. Borner MM, et alE. The detergent Triton X-100 induces a death pattern in human carcinoma cell lines that resembles cytotoxic lymphocyte-induced apoptosis. FEBS Lett. 1994 Oct 17;353(2):129-32.  [Content Brief]

  • [1]. Herman SM, et al. The hidden allergen: Triton X-100, a derivative of polyethylene glycol. J Allergy Clin Immunol Pract. 2021;9(7):2941.

    [2]. Ghosh R, et al. Unraveling diverse action of triton X-100 and methimazole on lysozyme fibrillation/aggregation: Physicochemical insights. Int J Biol Macromol. 2021;167:736-745.

    [3]. Colavita F, et al. Evaluation of the inactivation effect of Triton X-100 on Ebola virus infectivity. J Clin Virol. 2017 Jan;86:27-30.

    [4]. Koley D, et al. Triton X-100 concentration effects on membrane permeability of a single HeLa cell by scanning electrochemical microscopy (SECM). Proc Natl Acad Sci U S A. 2010 Sep 28;107(39):16783-7.

    [5]. Borner MM, et alE. The detergent Triton X-100 induces a death pattern in human carcinoma cell lines that resembles cytotoxic lymphocyte-induced apoptosis. FEBS Lett. 1994 Oct 17;353(2):129-32.

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Triton X-100 Related Classifications

  • Others
  • Biochemical Assay Reagents
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Triton X-1009002-93-1Triton X100Triton X 100Biochemical Assay Reagentsdetergentnonionic surfactantvaccinesmanufacturing processInhibitorinhibitorinhibit

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