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> Ultra Sensitive Rat Insulin ELISA kit

Ultra Sensitive Rat Insulin ELISA kit

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Referencia CC-90060

embalaje : 96tests

Marca : Crystal Chem

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Ultra Sensitive Rat Insulin ELISA kit

The Rat Insulin ELISA (Ultra Sensitive) is used to quantify levels of rat insulin in serum, plasma, cell culture, and fluid using only a 5 µL sample volume.
Highlights
  • Kits use only 5 µL sample
  • Dynamic range of 0.1- 64 ng/mL
  • High sensitivity (50 pg/mL) using 5 µL sample
For bulk purchases

Save money with our 10-kit pack

, available online!

Product Specifications

Catalog # 90060
Sample Size 5 µL
Sample Types Serum, plasma, cell culture, or fluid
Tests 96 wells (12 x 8 well strips)
Reagents In liquid form (except standard)
Sensitivity 0.05 ng/mL
Assay Range Low Range Assay: 0.1 - 6.4 ng/mL
Wide Range Assay: 0.1 - 12.8 ng/mL
High Range Assay: 1 - 64 ng/mL
Precision Intra-assay precision CV ≤ 10.0%
Inter-assay precision CV ≤ 10.0%
Storage 2-8°C
Regulatory Research Use Only
Distribution Worldwide
Assay Summary Assay and protocol summary

Additional Info

Crystal Chem's is a worldwide leading supplier of mouse and rat insulin ELISA kits. Our kits are the most sensitive on the market, and are used by leading research universities, pharma companies, and government institutions around the world.

Note: For bulk purchases, save money with our 10-kit pack.

Highlights:

Small Sample Volume Requires only a 5 µL sample, ideal for small quantity rat samples.
Highly Sensitive and Precise This assay has a sensitivity of 0.05 ng/mL using a 5 µL sample with CV ≤ 10.0%.
Wide Dynamic Range Range of 0.1 - 64.0 ng/mL, using only a 5 µL sample. Run both low and high insulin assays using the same kit.
Specific and Fast This is an easy-to-use kit that is highly specific for rat insulin with results in under 3.5 hours.



Specificity:

Substance Reactivity
Rat Insulin 100%
Mouse Insulin 100%*
Hamster Insulin Detected*
Rat C-peptide Not Detected
Rat pancreatic polypeptide Not Detected
Porcine glucagon Not Detected
Human Insulin like growth factor-I Not Detected
Human Insulin like growth factor-II Not Detected
*Can vary from lot to lot. Specific cross reactivity data is included with each kit



Typical Standard Curve:

References

Wojnacki, José, et al. “Tetraspanin-8 Sequesters Syntaxin-2 to Control Biphasic Release Propensity of Mucin Granules.” Nature Communications 2023; 14: 3710.  Read more
Yang, Jiufang, et al. “Intestinal Farnesoid X Receptor Modulates Duodenal Surface Area but Does Not Control Glucose Absorption in Mice.” International Journal of Molecular Sciences 2023; 24: 4132.  Read more
Frühbeck, Gema, et al. “Increased Aquaporin-7 Expression Is Associated with Changes in Rat Brown Adipose Tissue Whitening in Obesity: Impact of Cold Exposure and Bariatric Surgery.” International Journal of Molecular Sciences 2023; 24: 3412.  Read more
Tasić, Danica, et al. “Effects of Fructose and Stress on Rat Renal Copper Metabolism and Antioxidant Enzymes Function.” International Journal of Molecular Sciences 2022; 23: 9023.  Read more
Dahan, Tehila, et al. “Chronic Intermittent Hypoxia during Sleep Causes Browning of Interscapular Adipose Tissue Accompanied by Local Insulin Resistance in Mice.” International Journal of Molecular Sciences 2022; 23: 15462.  Read more
de Castro Barbosa, Thais, et al. "High-fat diet reprograms the epigenome of rat spermatozoa and transgenerationally affects metabolism of the offspring." Molecular Metabolism 2016; 5: 184-197.  Read more
Schlein, Christian, et al. "FGF21 lowers plasma triglycerides by accelerating lipoprotein catabolism in white and brown adipose tissues." Cell Metabolism 2016; 23: 441-453.  Read more
Lipina, Christopher, et al. "NEU3 sialidase as a marker of insulin sensitivity: Regulation by fatty acids." Cellular Signalling 2015; 27: 1742-1750.  Read more
Reynolds, Clare M., et al. "Maternal high‐fat diet‐induced programing of gut taste receptor and inflammatory gene expression in rat offspring is ameliorated by CLA supplementation." Physiological Reports 2015; 10: e12588.  Read more

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