ASM10552-STR-100 | VDCA-1 Antibody Clinisciences

Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-VDCA-1 Polyclonal Antibody (ASM10552). Tissue: Colon carcinoma cell line (RKO). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-VDCA-1 Polyclonal Antibody (ASM10552) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cytoplasm, Mitochondrion Outer Membrane, Cell Membrane. Magnification: 60X. (A) DAPI nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) VDCA-1 Antibody. (D) Composite.
Western blot analysis of Human HeLa and 293Trap cell lysates showing detection of ~30.7 kDa VDCA-1 protein using Rabbit Anti-VDCA-1 Polyclonal Antibody (ASM10552). Lane 1: Molecular Weight Ladder (MW). Lane 2: HeLa cell lysates. Load: 15 µg. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-VDCA-1 Polyclonal Antibody (ASM10552) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: ~30.7 kDa.

Product Information
Application Applications Legend: WB=Western Blot IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin-embedded Sections) IHC-F=Immunohistochemistry (Frozen Sections) IF=Immunofluorescence FC=Flow Cytopmetry IC=Immunochemistry ICC=Immunocytochemistry E=ELISA IP=Immunoprecipitation DB=Dot Blot CHIP=Chromatin Immunoprecipitation FA=Fluorescence Assay IEM=Immuno electron microscopy EIA=Enzyme Immunoassay	WB, ICC
Primary Accession	P21796
Other Accession	NP_003365.1
Host	Rabbit
Reactivity	Human
Clonality	Polyclonal
Description	Rabbit Anti-Human VDCA-1 Polyclonal
Target/Specificity	Detects ~37 kDa.
Other Names	N2441 Antibody, POR1 Antibody, Vdac1 Antibody, VDAC1 Antibody, MGC111064 Antibody, Outer mitochondrial membrane protein porin 1 Antibody, OMP2 Antibody, YNL055C Antibody, VDAC1_HUMAN Antibody, YNL2441C Antibody, VDAC-1 Antibody, Voltage dependent anion channel 1 Antibody, Voltage-dependent anion-selective channel protein 1 Antibody, Voltage dependent anion selective channel protein 1 Antibody, Plasmalemmal porin Antibody, Porin 31HL Antibody, Mitochondrial Porin Antibody, Porin 31HM Antibody, hVDAC1 Antibody, VDAC Antibody, VDAC1/porin Antibody, VDAC1 / Porin Antibody
Immunogen	Synthetic peptide from the N-terminal of Human VDCA-1
Purification	Peptide Affinity Purified
Storage	-20ºC
Storage Buffer	PBS, 50% glycerol, 0.09% sodium azide
Shipping Temperature	Blue Ice or 4ºC
Certificate of Analysis	A 1:1000 dilution of SPC-695 was sufficient for detection of VDCA-1 in 15 µg of human HeLa cell lysates by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.
Cellular Localization	Mitochondrion Outer Membrane \| Multi-Pass Membrane Protein \| Cell Membrane \| Multi-Pass Membrane Protein \| Membrane Raft \| Multi-Pass Membrane Protein

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