K2021-500tests | WST-1 Cell Proliferation Colorimetric Assay Kit Clinisciences

Background

Cell proliferation can be detected by a variety of methods. Cell proliferation induces an increase in the activity of the mitochondrial dehydrogenases, which cleaves the tetrazolium salt WST-1 to formazan.

The WST-1 Cell Proliferation Colorimetric Assay Kit provides a fast and sensitive way for quantification of cell proliferation and viability. Cell proliferation causes the increase in the amount of formazan dye formed that can be quantified by measuring the absorbance of the dye solution at 440 nm using microtiter plate reader. The kit can be used for the analysis of cell proliferation in response to pharmaceutical compounds, cytotoxic compounds like anticancer drugs and many other toxic agents. The assay can also be used for the detection of cell proliferation in response to cytokines, mitogens, growth factors and nutrients, etc. The assay is very easy and simple, requiring no solubilization, no washing and no harvesting steps, and is faster and more sensitive than MTS, MTT or XTT-based assays. The entire assay can be performed in a microtiter plate.

Quality Control

DataSheet

Related Biological Data

WST-1 Cell Proliferation Colorimetric Assay Kit

Related Biological Data

Overview

Kit components	WST-1 Developer Reagent Electron Mediator Solution
Detection method	Absorbance (420-480 nm)
Sample type	Cell culture (adherent and suspension)

Features & Properties

Features	Fast and convenient Simple one-step procedure; takes less than 4 hours. The method is so easy that it requires no washing/harvesting/or solubilization steps and is faster and more sensitive than MTT/XTT/or MTS-based assays. The entire assay can be performed directly in a 96-well plate.
Shipping	Gel pack
Storage Conditions	Store at -20°C.

Storage

Store at -20°C.

WST-1 Cell Proliferation Colorimetric Assay Kit

Referencia K2021-500tests

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Marca : APExBIO Technology

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Background

Quality Control

Related Biological Data

Related Biological Data

Related Biological Data

Overview

Features & Properties

Storage

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