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Zebrafish IL6 ELISA Kit

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Referencia orb1173535-24

embalaje : 24t

Marca : Biorbyt

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    Zebrafish IL6 ELISA Kit

    Catalog Number: orb1173535

    Catalog Numberorb1173535
    CategoryAssays and Kits
    DescriptionThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Interleukin 6(IL6). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Interleukin 6(IL6). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Interleukin 6(IL6), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Interleukin 6(IL6) in the samples is then determined by comparing the OD of the samples to the standard curve.
    Tested applicationsELISA
    ReactivityZebrafish
    Range7.82-500 pg/mL
    Concentration500 pg/mL
    Sensitivity3.2 pg/mL
    Protein Length3.5h
    Alternative namesIL-6, MGI2-A, MGI2A, HGF, BSF2, HSF, IFNB2, B-Cell
    NoteFor research use only
    Application notesStandard: 500 pg/mL. Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Zebrafish IL6. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Zebrafish IL6. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Zebrafish IL6, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Zebrafish IL6 in the samples is then determined by comparing the OD of the samples to the standard curve
    Sample Typesserum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
    Expiration DatePlease enquire.
    Zebrafish IL6 ELISA Kit