SDS Precast gels
Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis (SDS-PAGE) is a pivotal technique in molecular biology and biochemistry for the separation of proteins based on their molecular weight. This methodology employs polyacrylamide gels that facilitate the migration of denatured proteins through a mesh-like matrix, allowing researchers to analyze protein samples with high precision. Precast gels, as opposed to hand-poured gels, offer several advantages including consistency, ease of use, and reduced preparation time, making them a popular choice in laboratories.
Applications of SDS Precast Gels
SDS-PAGE is extensively utilized in various fields including:
- Protein Analysis: For determining the purity and molecular weight of proteins.
- Western Blotting: Following electrophoresis, proteins can be transferred to membranes for further analysis using specific antibodies.
- Enzyme Activity Studies: Native PAGE variants allow researchers to study enzyme activities while maintaining the native state of the proteins.
