SDS precast gels gradient 12%
SDS precast gels with a 12% gradient are pivotal in the field of protein electrophoresis, providing researchers with a reliable method for the separation and analysis of proteins based on their molecular weight. These gels are characterized by their ability to create a gradient of polyacrylamide concentration, which allows for the effective resolution of proteins that vary significantly in size.
Advantages of SDS Precast Gels
- Convenience and Consistency: Precast gels are ready-to-use and eliminate the variability associated with hand-casting gels. This ensures greater reproducibility and more stringent quality control, which is crucial for experimental accuracy
- Optimized Separation: The gradient structure of these gels facilitates the separation of a wide range of protein sizes within a single run. Larger pores at the top of the gel accommodate larger proteins, while smaller pores at the bottom restrict smaller proteins, allowing for efficient separation across a broad spectrum of molecular weights
- Compatibility: Many SDS precast gels are designed to be compatible with popular electrophoresis systems, such as those from Bio-Rad and Invitrogen. This compatibility ensures that researchers can easily integrate these gels into their existing workflows without the need for additional equipment or modifications
Applications
SDS precast gels with a 12% gradient are widely used in various applications, including:
- SDS-PAGE: For analyzing protein samples in denaturing conditions.
- Western Blotting: Following electrophoresis, proteins can be transferred to membranes for further analysis.
- Protein Purification: Assisting in the purification process by separating proteins based on size.
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NG10-012
10/pk
NG15-012
10/pk
NG12-012
10/pk
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