Acetyl CoA synthetase (ACSS2) Rabbit Polyclonal Antibody

Referencia AP20096BT-N

embalaje : 1ml

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Acetyl CoA synthetase (ACSS2) Rabbit Polyclonal Antibody

Specifications
Product Data
Application ELISA, ID, IF, IP, R, WB
Application This product is intended for use in precipitating and non-precipitating antibody-binding assays such as e.g., ELISA and Western blotting and Immunofluorescence or Histochemical techniques (1/1,000-1/10,000).
Reactivity Bakers Yeast
Antibody Host Rabbit
Isotype IgG
Clonality Polyclonal
Immunogen Purified S-Acetyl Coenzyme A Synthetase from baker's Yeast
Specificity The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: Immunoelectrophoresis, Cross-Immunoelectrophoresis, single Radial Immunodiffusion (Ouchterlony), block titration, ELISA, Immunoblotting and Enzyme Inhibition.
Cross-reactivities against enzymes of other sources may occur but have not been determined.
Buffer PBS, pH 7.2 without preservatives and foreign proteins.
Label: Biotin
State: Lyophilized IgG fraction.
Label: Conjugation Procedure: A proprietary technique for the binding to biotin is used, followed by several purification steps. After each step activity and specificity are tested in a variety of techniques. The conjugate is lyophilized to assure stability and long shelf life
Molar radio: ~5.5
Reconstitution Method Restore by adding 1.0 ml of sterile distilled water.
Concentration lot specific
Purification Ammonium Sulphate Precipitation and Ion Exchange Chromatography.
Conjugation Biotin
Storage Store the antibody lyophilized at 2-8°C and reconstituted at 2-8°C for one week or (in aliquots) at -20°C for longer.
If a slight precipitation occurs upon storage, this should be removed by centrifugation.
Stability Shelf life: one year from despatch.
Gene Name acyl-CoA synthetase short-chain family member 2
Database Link
Background Acetyl Coenzyme A Synthetase is a cytosolic enzyme that catalyzes the activation of acetate for use in lipid synthesis and energy generation. It acts as a monomer and produces acetyl-CoA from acetate in a reaction that requires ATP. Expression of this gene is regulated by sterol regulatory element-binding proteins, transcription factors that activate genes required for the synthesis of cholesterol and unsaturated fatty acids. Two transcript variants encoding different isoforms have been found for this gene.
Synonyms ACAS2, ACSA
Reference Data
Protein Pathways Glycolysis / Gluconeogenesis, Metabolic pathways, Propanoate metabolism, Pyruvate metabolism
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