CD81 (B-11)

Referencia sc-166029

embalaje : 200µg/ml

Marca : Santa Cruz Biotechnology

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Anticorps CD81 (B-11): sc-166029

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Fiches techniques
  • L'anticorps CD81 B-11 est un monoclonal IgG2b κ L'Anticorps CD81, cité dans 371 publications, fourni en 200 µg/ml
  • dirigé contre les acides aminés 90-210 de CD81 d'origine human
  • L'Anticorps CD81 (B-11) est recommandé pour la détection de CD81 d'origine mouse, rat et human par WB, IP, IF, IHC(P) et ELISA
  • Anti-L'Anticorps CD81 (B-11) est disponible conjugué à l'agarose pour IP; à l'HRP pour WB, IHC(P) et ELISA; et soit à la phycoerythrin ou FITC pour IF, IHC(P) et FCM
  • aussi disponible conjugué à l'Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 ou Alexa Fluor® 647 pour WB (RGB), IF, IHC(P) et FCM
  • aussi disponible conjugué à l'Alexa Fluor® 680 ou Alexa Fluor® 790 pour WB (NIR), IF et FCM
  • 2b BP-HRP">m-IgG2b BP-HRP et m-IgGκ BP-HRP sont les réactifs secondaires recommandés pour la détection de l'anticorps CD81 (B-11) pour des applications de WB and IHC(P). Ces réactifs sont maintenant proposés en kit avec l'anticorps CD81 (B-11) ().

ACCÈS RAPIDE AUX LIENS

or CD81 Activators for functional analysis of cellular responses to CD81

Learn more about our ImmunoCruz® Antibody Conjugates and Cruz Marker™ MW Standards Promotional

L'anticorps CD81 (B-11) est un anticorps monoclonal de souris IgG2b κ CD81 (également appelé anticorps TAPA1) qui détecte la protéine CD81 de souris, de rat et d'origine humaine par WB, IP, IF, IHC(P) et ELISA. L'anticorps CD81 (B-11) est disponible sous forme d'anticorps anti-CD81 non conjugué, ainsi que sous plusieurs formes conjuguées d'anticorps anti-CD81, y compris agarose, HRP, PE, FITC et plusieurs Alexa Fluor® conjugués. CD81, également appelé TAPA-1, est une protéine transmembranaire de type III qui est largement exprimée sur les cellules d'origine hématopoïétique, neuroectodermique et mésenchymateuse.

Pour la Recherche Uniquement. Non destiné à un usage diagnostique ou thérapeutique.

Alexa Fluor® est une marque déposée de Molecular Probes Inc., OR., USA

LI-COR® et Odyssey® sont marques déposées de LI-COR Biosciences

Informations pour la commande

Nom du produitRef. CatalogueCOND.Prix HTQTÉFavoris

Anticorps CD81 (B-11)

sc-166029
200 µg/ml
.00

CD81 (B-11): m-IgGκ BP-HRP Kit

sc-521489
200 µg Ab, 40 µg BP
.00

CD81 (B-11): m-IgG2b BP-HRP Kit

sc-548913
200 µg Ab; 10 µg BP
.00

Anticorps CD81 (B-11) AC

sc-166029 AC
500 µg/ml, 25% agarose
.00

Anticorps CD81 (B-11) HRP

sc-166029 HRP
200 µg/ml
.00

Anticorps CD81 (B-11) FITC

sc-166029 FITC
200 µg/ml
.00

Anticorps CD81 (B-11) PE

sc-166029 PE
200 µg/ml
.00

Anticorps CD81 (B-11) Alexa Fluor® 488

sc-166029 AF488
200 µg/ml
.00

Anticorps CD81 (B-11) Alexa Fluor® 546

sc-166029 AF546
200 µg/ml
.00

Anticorps CD81 (B-11) Alexa Fluor® 594

sc-166029 AF594
200 µg/ml
.00

Anticorps CD81 (B-11) Alexa Fluor® 647

sc-166029 AF647
200 µg/ml
.00

Anticorps CD81 (B-11) Alexa Fluor® 680

sc-166029 AF680
200 µg/ml
.00

Anticorps CD81 (B-11) Alexa Fluor® 790

sc-166029 AF790
200 µg/ml
.00

Does CD81 antibody (cat #166029) require non-reducing condition or reducing condition for western blot?

Posée par: dustn
Thank you for your question. This antibody recognizes both native and denatured CD81 protein.
Répondue par: Tech Support Europe
Date de publication: 2022-11-30

Hello I would like to know the ratio of fluorochrom per antibody in the Anticorps CD81 (B-11) Alexa Fluor® 647 ; and also the ratio in his isotype ctrl IG g1 anti mouse AF647. Thanks

Posée par: leacurie
Thank you for your question. It would be helpful if you could call us, allowing for a more interactive discussion of this and other related questions.
Répondue par: Technical Support
Date de publication: 2021-07-22

What westernblot protocol should I use? reducing or non-reducing? I cannot find the information on the sample data sheet. I tried the reducing protocol and get nice single band, but the size is smaller than 20kDa. Thank you

Posée par: yan DK
Thank you for your question. Please contact your local distributor or technical service for further assistance.
Répondue par: Dallas Tech Service
Date de publication: 2021-04-20

Bonjour , Avez vous la masse molaire de cet Ac?

Posée par: Barbara B
Merci puor votre question. La masse molaire de cet anticorps est 150.000 g/mol.
Répondue par: Tech Support Europe
Date de publication: 2020-11-27

如何领取试用装

Posée par: Anonyme
感谢您的提问。如何获取免费试用装,请联系我们的技术支持部门。亚洲技术支持电话(+86) 021-60936350、邮箱asia@scbio.cn、或选择网页上Live Chat中的“中文支持"。 Thank you for your question. Please contact our Technical Service Department for more information on how to receive a free 10 ug sample of this monoclonal antibody. Our Asian Technical Service team is available at (+86) 021-60936350 or asia@scbio.cn or by live chat on our website.
Répondue par: Technical Support 9
Date de publication: 2019-03-26

I plan to buy CD81 antibody sc166029 to detect exosome.Can I have some sample for this?

Posée par: Naoya
Please contact our Technical Service Department for more information on how to receive a free 10 ug sample of this monoclonal antibody.
Répondue par: Technical Support
Date de publication: 2019-02-02

What is the primary antibody dilution range that we use for CD81?

Posée par: Deepika
Thank you for your question. The dilution range is dependent on the application. Please find the dilution range in the product datasheet for each application, which can be downloaded from this link: https://datasheets.scbt.com/sc-166029.pdf
Répondue par: Technical Support
Date de publication: 2019-01-06

How many FITC molecules are conjugated to each antibody ? and What is the total molecular weight of CD81 Antibody (B-11) FITC (sc-166029 FITC ) please?

Posée par: AgaS
Thank you for your question. The molecular weight of an antibody is ~150 kDa. The ratio is ~4-7 FITC : antibody. The expected molecular weight of CD81 is ~21-26kDa. If you have any further questions, please contact our European Technical Service team. You can reach them by phone at Toll Free: +49 6221 4503 0, by email at: europe@scbt.com or by live chat directly on our website, www.scbt.com
Répondue par: Tech Service
Date de publication: 2017-06-28
  • Anticorps CD81 (B-11) Questions - page 2
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Rated 5 de 5 de par Very good to detect CD81 in exosome extractsWe used CD81 antibody to detect the protein in exosomes isolated from the Bj5ta cell line and worked very well at 1:200 dilution (western blot).
Date de publication: 2023-07-17
Rated 5 de 5 de par Very good to detect CD81 in exosome extractsWe used CD81 antibody to detect the protein in exosomes isolated from the Bj5ta cell line and worked very well at 1:200 dilution (western blot).
Date de publication: 2023-07-17
Rated 5 de 5 de par Best CD81 antibodysc-166029 (1:500) is by far the best CD81 antibody
Date de publication: 2023-07-11
Rated 4 de 5 de par CD81 in exosomes obtained from plasmaWe used this antibody to detect CD81 protein bu WB in extract obtained from exosomes isolated from plasma samples and it worked at 1:200 dilution.
Date de publication: 2023-05-18
Rated 4 de 5 de par It detects CD81 on exosome extracts by WBWe used this Ab to identify the presence of CD81 as a marker of exosomes by Western blot using protein extracts from ultracentrifigation pellets and it worked remarkably well
Date de publication: 2021-11-19
Rated 3 de 5 de par HTLV-1感染細胞由来エクソソームのCD81をWBで検出5%スキムミルクを使ってブロッキングを行うとバックグラウンドが高く、バンドを検出できませんでした。市販のブロッキング剤を用いるとバンドが検出できました。
Date de publication: 2021-09-09
Rated 5 de 5 de par Works very well on MCF7-derived exosomesI tested this antibody on exosomes purified by ultracentrifugation from MCF7 extracellular medium. It produces a single band at the expected size and normal ECL reagent was sufficient to detect it.
Date de publication: 2021-03-26
Rated 4 de 5 de par Validated for equine useI have used this antibody for ICC of equine endothelial cells (4ug/ml) and investigating exosomes from equine plasma in an ELISA (1ug/ml). Worked well in both situations.
Date de publication: 2020-08-13
  • Anticorps CD81 (B-11) Avis - page 2
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Anticorps CD81 (B-11) est évalué 4.6 de 5 de 30.
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