CD95 (FAS) Mouse Monoclonal Antibody [Clone ID: ZB4]
Specifications
Product Data | |
Clone Name | ZB4 |
Applications | FC, Neutralize, WB |
Recommended Dilution | Western blot: 5 μg/ml for chemiluminescence detection system. Flow cytometry: 5-20 μg/ml (final concentration). Neutralizing activity: This clone ZB4 inhibits the anti-Fas antibody (clone CH-11) -induced apoptosis when the cells are pretreated with ZB4 for 1 hour at a concentration of 10-500 ng/ml in medium. Detailed procedure is provided in the protocols below. |
Reactivities | Human |
Host | Mouse |
Isotype | IgG1 |
Clonality | Monoclonal |
Immunogen | Recombinant human Fas |
Specificity | This antibody reacts with Fas. |
Formulation | PBS containing 50% glycerol, pH 7.2 State: Azide Free State: Liquid Ig fraction containing no preservatives |
Concentration | lot specific |
Purification | Protein A agarose |
Conjugation | Unconjugated |
Storage | Store (in aliquots) at -20 °C. Avoid repeated freezing and thawing. |
Stability | Shelf life: one year from despatch. |
Gene Name | Fas cell surface death receptor |
Database Link | |
Background | It is now widely accepted that apoptosis plays an important role in the selection of immature thymocytes and Ag-primed peripheral T cells. Fas (also known as CD95/APO-1) is a cell surface protein belonging to the tumor necrosis factor receptor superfamily, which is expressed in a variety of normal and neoplastic cells. Binding of FasL to Fas or crosslinking of Fas by anti-Fas monoclonal antibodies results in rapid induction of apoptosis in Fas expressing cells. Clone ZB4 is known as Fas blocking, Fas neutralizing or Fas antagonistic antibody, whereas anti-human Fas antibody (clone CH-11) is known as the agonistic antibody. |
Synonyms | FASLG receptor, Apo-1 antigen, APT1, FAS1, TNFRSF6 |
Note | This product was originally produced by MBL International. Protocol: SDS-PAGE & Western Blotting Flow cytometric analysis for floating cells Flow cytometric analysis for whole blood cells Neutralizing activity |
Reference Data |
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