K2135-100 assays | D-Sorbitol Colorimetric Assay Kit Clinisciences

Background

Sorbitol is a sugar alcohol and often used as an artificial sweetener and a laxative. Sorbitol can also be used as a humectant and thickening agent in cosmetics. Sorbitol is naturally produced in a variety of fruits and can be produced in small amounts in humans via the reduction of glucose by aldose reductase. Sorbitol can be used to screen the O154:H7 strain of E. coli, since it cannot metabolize sorbitol. Because of its poor ability to diffuse across the cell membrane, sorbitol is one of the causes of damage in diabetes.

The D-Sorbitol Colorimetric Assay Kit provides a simple, fast and convenient way for detection of sorbitol in various samples such as fruit juices, fruits, foods, pharmaceuticals, cosmetics, paper and some other biological samples based on colorimetric method. In the assay, sorbitol is oxidized to fructose with the proportional intense color (λ = 560 nm) development. The kit can detect 0.1-10 nmol sorbitol per sample.

Features & Properties

Features	Fast and convenient; Simple procedure; Takes approx. 35-40 minutes; The kit provides an accurate assay for measuring sorbitol in various samples.
Shipping	Blue Ice
Storage Conditions	Store at -20°C.

Quality Control

DataSheet

Storage

Store at -20°C.

Overview

Kit components

Sorbitol Assay Buffer, Sorbitol Probe, Sorbitol Enzyme Mix, Sorbitol Developer, Sorbitol Standard (100 mM)

D-Sorbitol Colorimetric Assay Kit

Referencia K2135-100 assays

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