Gja1 (NM_010288) Mouse Untagged Clone
Referencia MC205621
embalaje : 10ug
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Gja1 (NM_010288) Mouse Untagged Clone
| Product Data | |
| Target Symbol | Gja1 |
|---|---|
| Synonyms | AU042049; AW546267; Cnx43; connexin43; Cx43; Cx43alpha1; Gja-1; Npm1 |
| Vector | PCMV6-Kan/Neo |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | Fully Sequenced ORF >BC006894 CAGCACTTTTCTTTCATTGGGGGAAAGGCGTGAGGGAAGTACCCAACAGCAGCAGACTTTGAAACTTTAA ACAGACAGGTCTGAGAGCCCGAACTCTCCTTTTCCTTTGACTTCAGCCTCCAAGGAGTTCCACCACTTTG GCGTGCCGGCTTCACTTTCATTAAGTGAAAGAGAGGTGCCCAGACATGGGTGACTGGAGCGCCTTGGGGA AGCTGCTGGACAAGGTCCAAGCCTACTCCACGGCCGGAGGGAAGGTGTGGCTGTCGGTGCTCTTCATTTT CAGAATCCTGCTCCTGGGGACAGCGGTTGAGTCAGCTTGGGGTGATGAACAGTCTGCCTTTCGCTGTAAC ACTCAACAACCCGGTTGTGAAAATGTCTGCTATGACAAGTCCTTCCCCATCTCTCACGTGCGCTTCTGGG TCCTTCAGATCATATTCGTGTCTGTGCCCACACTCCTGTACTTGGCTCACGTGTTCTATGTGATGAGAAA GGAAGAGAAGCTGAACAAGAAAGAAGAGGAGCTCAAAGTGGCGCAGACCGACGGGGTCAACGTGGAGATG CACCTGAAGCAGATTGAAATCAAGAAGTTCAAGTATGGGATTGAAGAACACGGCAAGGTGAAGATGAGAG GTGGCCTGCTGAGAACCTACATCATCAGCATCCTCTTCAAGTCTGTCTTCGAGGTGGCCTTCCTGCTGAT CCAGTGGTACATCTATGGGTTCAGCCTGAGTGCGGTCTACACCTGCAAGAGAGATCCCTGCCCCCACCAG GTGGACTGCTTCCTCTCACGTCCCACGGAGAAAACCATCTTCATCATCTTCATGCTGGTGGTGTCCTTGG TGTCTCTCGCTCTGAATATCATTGAGCTCTTCTATGTCTTCTTCAAGGGCGTTAAGGATCGCGTGAAGGG AAGAAGCGATCCTTACCACGCCACCACCGGCCCACTGAGCCCATCCAAAGACTGCGGATCTCCAAAATAT GCTTACTTCAATGGCTGCTCCTCACCAACGGCCCCACTCTCACCTATGTCTCCTCCTGGGTACAAGCTGG TCACTGGTGACAGAAACAATTCCTCCTGCCGCAATTACAACAAGCAAGCCAGCGAGCAAAACTGGGCGAA TTACAGCGCAGAGCAAAATCGAATGGGGCAGGCCGGAAGCACCATCTCCAACTCCCACGCCCAGCCGTTT GATTTCCCTGACGACAGCCAAAATGCCAAAAAAGTTGCTGCTGGACACGAACTCCAGCCCTTAGCTATCG TGGATCAGCGACCTTCCAGCAGAGCCAGCAGCCGCGCCAGCAGCAGACCTCGGCCTGATGACCTGGAGAT TTAAACAGGCTTGAACATCAAGCTGCCAATCGATTGTGGAGGAGAAAAAAAAGGGTGCTTGCAGAACGTG CACCTGGGGTGTTCATTTCGTTCCCGTGGAGGTGGTACTCAACAACCTCAGTAATGAGGCGTAGAAAACA AAGACATTACAATATCTAGGTTCCTTGGGGGGTGTTTTGGGATAGCTAGGCGGCAAAAGTAGGGAAAGGG GAGGTATGTAACGGTATTTAATGTAGAAGATTCAAAGAGCTTAAATTCTAGTAAGAGTCTCATTGGATGA AACATAGATAGGGCTTTCTCTCTCTGCCCCCCAACTGAACCTTAAGAATGGTTCTGTATACATGAGTGAG TGGGTGATATATATTTTTTTTAATTTTTGTTTTACTGAGATTCTGCCATAGAGCTTTGAGCAGGAATCCA AGTCCTCAACATGGCATTTCCTTTATGAAAAGACAGGTTGTCCTACATCCCCGCTAAAAAACATTCCAGT GTTTAAAAACTTGGCAGTTTGCAGGCGAGCTTCCCTGGCCTGACCCTCTAGGTGTGGATGGACCTTATGC TACTATACACGATTTTCATTCTTGGTAGGTATCAATTCGAAGTTCAGACAAGGTTCAAAGAAAAAGATTG CCCATGTATTTGCATCTCAGTGGGTTCTTTTTCAAATCTGTCCCACCTTTGTGTCTTCCATATATTATCC TCAGCTGGTCCTCACCCTCACCAAATGATTTCTATCGACATTTTTAAAACAGTGAGAAAGTCTTTTTTTT TTTTTTTTGAGTTAGCATCAGGGAGGCAAGCCATGCTCAATATTTAACAATCGCTTCTGTCTATGTGTGG GTGTGCAAGTGTGTAAGCGTGTGTTTTGTCATTATTGGTACAAGCAGAGGCAGTATAAACTCACAGATTT GAATCGAATTCACACAGTGTTCAAATTTGAACCTTCCTCATGGATCTTTGTGGTGTGGGCCAACGTGGTG TTTACATTATAGAATTCCTGCCGTGCAAAAGTGTAAAGCACACACTTTTTCCCTAAAATATTTTTTCCAC GTATCCTATTATGGATACTGGTTTTGTTAATTATGATTTTTTTTTCTTTTTTAGAATGTAGCAGTAATAG CCATTACTGAAATGAATGATTTCCTTTTTCTGAAATATAATCATTGATGCTTGAATGATAGAATTTTAGT ACTGTAAACAGGCTTTAGTCATTAATGTGAGAGACTTAGAAGAGGGTTGCTTAGAGTGGACTATCAAGTG AGCCTAAAGGAACTTTGTAGTAACTGGTAATCTGGTAATTTTTGTCCTACTTAACTACACATTAACTCAG AACTTGTATTCTGAGTTTAACAGTCTTTTAGATTGACGAGCAACTTGGATGTTTGCACTAAGATTTTCTT TGAGATACTAGAGGGGGTGAAGGAGTTTTCAGCAGTGCACATGTAACTAATTTATTTGAACTGTAAGCTA AAGACACCTACCAGTTTCTTCAAGTGACTTAAAAAAACTCATCACAGATGATTGAAATGTCGAGTTATCA TGTTTCCTCTTGCGCGCCAGCTACACAAGGAGTTTTTGGACAATGAGAAACTAATTTGTTTGACATTCCA TGTTAAACTACTGTCATGTTCAGCTTCATTGCATGTAATGTAGACCTAGCCCATCCAATCAATGTGCTCG GGAAAGTGTTCTTTATTCAATAAAATTTTAATTTAGTATAATAAAAAAAAAAAAAAAAAAA |
| Restriction Sites | RsrII-NotI Plasmid Map |
| ACCN | NM_010288 |
| Insert Size | 1149 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at info@clinisciences.com The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC006894, AAH06894 |
| RefSeq Size | 3071 bp |
| RefSeq ORF | 1149 bp |
| Locus ID | 14609 |
| UniProt ID | P23242 |
| Cytogenetics | 10 28.64 cM |
| Summary | Gap junction protein that acts as a regulator of bladder capacity. A gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell. Negative regulator of bladder functional capacity: acts by enhancing intercellular electrical and chemical transmission, thus sensitizing bladder muscles to cholinergic neural stimuli and causing them to contract. May play a role in cell growth inhibition through the regulation of NOV expression and localization (PubMed:15181016). Plays an essential role in gap junction communication in the ventricles (PubMed:26403541).[UniProtKB/Swiss-Prot Function] |
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