Granulocyte Macrophage Colony Stimulating Factor, Recombinant, Mouse (GM-CSF, Colony-stimulating Factor, CSF, Csf2, Csfgm, MGI-IGM)

GM-CSF is a hematopoietic growth factor that stimulates the development of neutrophils and macrophages, and promotes the proliferation and development of early erythroid megakaryocytic and eosinophilic progenitor cells. It is produced in endothelial cells, monocytes, fibroblasts and T-lymphocytes. GM-CSF inhibits neutrophil migration and enhances the functional activity of the mature end-cells. The human and murine molecules are species-specific and exhibit no cross-species reactivity. Recombinant mouse GM-CSF is a 14.2kD globular protein consisting of 125aa residues.||Recombinant protein corresponding to mouse GM-CSF, expressed in E.coli.||Biological Activity:|The ED50 as determined by the dose-dependent stimulation of the proliferation of murine FDC-P1 cells is ≤0.05ng/ml, corresponding to a specific activity of ≥ 2x10e7 units/mg.||Amino Acid Sequence:|MAPTRSPITV TRPWKHVEAI KEALNLLDDM PVTLNEEVEV VSNEFSFKKL TCVQTRLKIF EQGLRGNFTK LKGALNMTAS YYQTYCPPTP ETDCETQVTT YADFIDSLKT FLTDIPFECK KPVQK||Storage and Stability:|Lyophilized and reconstituted products are stable for 6 months after receipt at -20°C. Reconstitute with sterile acetic acid. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.