Human IgG3 (F(ab)2 specific) Mouse Monoclonal Antibody [Clone ID: NI 330 (HP 6050)]

Specifications

Product Data
Clone Name	NI 330 (HP 6050)
Applications	ELISA, IF, IP, WB
Recommended Dilution	To identify the presence of IgG3 in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, indirect Immunoperoxidase staining of cytoplasmic IgG3, and immunoblotting using a peroxidase labelled monoclonal antibody against FITC. General Recommended Dilutions: Histochemical Use: 1/10-1/50 ELISA: from 1/200 upwards Western blot: from 1/400 upwards
Reactivities	Human
Host	Mouse
Isotype	IgG1
Clonality	Monoclonal
Immunogen	Highly purified monoclonal IgG3 isolated from pooled human serum.
Specificity	The reactivity of the antiserum is restricted to a subclass specific determinant on the F(ab’)2 part of the IgG3 molecule as tested in direct binding enzyme immunoassay, immunoblotting, immunoprecipitation and direct immunoperoxidase staining of cytoplasmic Ig. The antibody does not react with any other component of the human immunoglobulin system or any other human plasma protein as tested. This antiserum has not been tested for cross-reactivity with other species.
Formulation	PBS, pH 7.2 without preservatives and foreign proteins Label: FITC State: Lyophilized purified IgG fraction Label: Fluorescein Isothiocyanate Absorption emission: 492 nm / 515 nm Molar radio: Fluorochrome/IgG ~1.0
Reconstitution Method	Restore by adding 0.5 ml sterile distilled water
Concentration	0.4 mg/ml
Conjugation	FITC
Storage	Store the antibody lyophilized at 2-8°C for one month or (in aliquots) at -20°C for longer. Diluted ascites should be stored at 2-8°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. Avoid repeated thawing and freezing.