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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Application

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human lymphoid tissue with Human IgG3 monoclonal antibody, clone RM119 (Biotin) (Cat # MAB14958).

Enzyme-linked Immunoabsorbent Assay
Application

Enzyme-linked Immunoabsorbent Assay

ELISA analysis of Human IgG3 monoclonal antibody, clone RM119 (Cat # MAB12820) at the following concentrations: 0.2 ug/mL, 0.05 ug/mL, 0.01 ug/mL. The plate was coated with 50 ng/well of different immunoglobulins and the result shows this antibody reacts to human IgG3; no cross reactivity human IgG1, IgG2, IgG4, IgE, IgA, or IgD, mouse IgG, rat IgG, or goat IgG. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.

  • Specifications

    Product Description

    Rabbit recombinant monoclonal antibody raised against human IgG3.Recombinant Antibody,Recombinant Antibodies,Recombinant Monoclonal Antibody,RecomAb,Recombinant Ab,Recombinant Monoclonal Antibodies,Recombinant Abs

    Antibody Species

    Rabbit

    Immunogen

    Original antibody is raised against human IgG3.

    Sequence

    N/A

    Reactivity

    Human

    Form

    Liquid

    Conjugation

    Biotin

    Purification

    Protein A purification

    Isotype

    IgG

    Recommend Usage

    ELISA (0.05-0.2 ug/well for capture, 0.05-0.2 ug/mL for detection)
    Flow Cytometry
    Immunocytochemistry (0.5-2 ug/mL)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (0.5-2 ug/mL)
    The optimal working dilution should be determined by the end user.

    Storage Buffer

    In PBS (50% glycerol, 1% BSA, 0.09% sodium azide)

    Storage Instruction

    Store at -20°C.
    Aliquot to avoid repeated freezing and thawing.

    Note

    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

  • Applications

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

    Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human lymphoid tissue with Human IgG3 monoclonal antibody, clone RM119 (Biotin) (Cat # MAB14958).

    Immunocytochemistry

    Enzyme-linked Immunoabsorbent Assay

    ELISA analysis of Human IgG3 monoclonal antibody, clone RM119 (Cat # MAB12820) at the following concentrations: 0.2 ug/mL, 0.05 ug/mL, 0.01 ug/mL. The plate was coated with 50 ng/well of different immunoglobulins and the result shows this antibody reacts to human IgG3; no cross reactivity human IgG1, IgG2, IgG4, IgE, IgA, or IgD, mouse IgG, rat IgG, or goat IgG. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.

    Flow Cytometry

  • Gene Info — IGHG3

    Entrez GeneID

    3502

    Protein Accession#

    P01860

    Gene Name

    IGHG3

    Gene Alias

    DKFZp686H11213, FLJ39988, FLJ40036, FLJ40253, FLJ40587, FLJ40789, FLJ40834, IgG3, MGC45809

    Gene Description

    immunoglobulin heavy constant gamma 3 (G3m marker)

    Omim ID

    147120

    Gene Ontology

    Hyperlink

    Other Designations

    -

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