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Western Blot (Cell lysate)
Application

Western Blot (Cell lysate)

IDH1 polyclonal antibody (Cat # PAB19472)(0.5 ug/mL) staining of S. cerevisiae lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

  • Specifications

    Product Description

    Goat polyclonal antibody raised against synthetic peptide of IDH1.

    Immunogen

    A synthetic peptide corresponding to internal region of Saccharomyces cerevisiae (S288c) IDH1.

    Sequence

    C-EPGSRHVGLDIKGQN

    Host

    Goat

    Theoretical MW (kDa)

    39.3

    Reactivity

    Yeast

    Form

    Liquid

    Purification

    Ammonium sulfate precipitation

    Recommend Usage

    ELISA (1:1600)
    Western Blot (0.3-1 ug/mL)
    The optimal working dilution should be determined by the end user.

    Storage Buffer

    In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)

    Storage Instruction

    Store at -20°C.
    Aliquot to avoid repeated freezing and thawing.

    Note

    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

  • Applications

    Western Blot (Cell lysate)

    IDH1 polyclonal antibody (Cat # PAB19472)(0.5 ug/mL) staining of S. cerevisiae lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

    Enzyme-linked Immunoabsorbent Assay

  • Gene Info — IDH1

    Entrez GeneID

    855691

    Protein Accession#

    NP_014361.1

    Gene Name

    IDH1

    Gene Alias

    -

    Gene Description

    Idh1p

    Gene Ontology

    Hyperlink

    Gene Summary

    which catalyzes the oxidation of isocitrate to alpha-ketoglutarate in the TCA cycle

    Other Designations

    Subunit of mitochondrial NAD(+)-dependent isocitrate dehydrogenase, which catalyzes the oxidation of isocitrate to alpha-ketoglutarate in the TCA cycle

  • Publication Reference