IgG1 Isotype Control (FITC)

Referencia 590304-100ug

embalaje : 100ug

Marca : US Biological

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590304 IgG1 Isotype Control (FITC)

Clone Type
Polyclonal
Host
mouse
Isotype
IgG1
Grade
Affinity Purified
Applications
FC
Shipping Temp
Blue Ice
Storage Temp
4°C Do Not Freeze

Isotype matched controls are useful in flow cytometry to determine a gross estimate of non-specific binding that may be observed with a specific antibody of interest. In general, an isotype control should be of the same species and isotype as the antibody of interest, used at the same concentration, and conjugated to the same fluorophore. ||Applications: |Suitable for use in Flow Cytometry and Immunofluorescence Microscopy. Other applications not tested.||Recommended Dilutions:|Optimal dilutions to be determined by the researcher.||Storage and Stability:|Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Applications
Product Type: Mab|Isotype: IgG1|Clone No: MOPC-21|Host: mouse|Concentration: As Reported |Form: Supplied as a liquid in PBS, 0.09% sodium azide.|Purity: Purified by Protein G affinity chromatography.|Specificity: Recognizes IgG1.||Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Form
Supplied as a liquid in TBS, pH 7.4, 50% glycerol. Labeled with Fluorescein isothiocyanate (FITC).
Purity
Purified by Protein G affinity chromatography.
References
1. The CD24hi smooth muscle subpopulation is the predominant fraction in uterine fibroids: M. Drosch, et al.; Mol. Hum. Reprod. 20, 664 (2014), Abstract;|Vasculogenic Conditioning of Peripheral Blood Mononuclear Cells Promotes Endothelial Progenitor Cell Expansion and Phenotype Transition of Anti-Inflammatory Macrophage and T Lymphocyte to Cells With Regenerative Potential: H. Masuda, et al.; JAHA 3, e000743 (2014), Application(s): Flow cytometry analysis of human peripheral blood mononuclear cells (PBMNCs), Abstract; Full Text|Altered expression of TNF-alpha signaling pathway proteins in systemic lupus erythematosus: L.J. Zhu, et al.; J. Rheumatol. 37, 1658 (2010), Abstract;|Monocyte heterogeneity underlying phenotypic changes in monocytes according to SIV disease stage: W.K. Kim, et al.; J. Leukoc. Biol. 87, 557 (2010), Application(s): Flow cytometry analysis of rhesus macaques peripheral blood monocytes (PBMCs), Abstract; Full Text|Expanded Population of Activated Antigen-Engaged Cells within the Naive B Cell Compartment of Patients with Systemic Lupus Erythematosus: N.H. Chang, et al.; J. Immunol. 180, 1276 (2008), Application(s): Flow cytometry analysis of human peripheral blood monocytes (PBMCs), Abstract; Full Text|Inhibition of Invariant Chain Processing, Antigen-Induced Proliferative Responses, and the Development of Collagen-Induced Arthritis and Experimental Autoimmune Encephalomyelitis by a Small Molecule Cysteine Protease Inhibitor: P.L. Podolin, et al.; J. Immunol. 180, 7989 (2008), Application(s): Flow cytometry analysis of human B cell line Raji, Abstract; Full Text|The effects of dasatinib on IgE receptor-dependent activation and histamine release in human basophils: M. Kneidinger, et al.; Blood 111, 3097 (2008), Abstract; Full Text|Nonfucosylated therapeutic IgG1 antibody can evade the inhibitory effect of serum immunoglobulin G on antibody-dependent cellular cytotoxicity through its high binding to FcγRIIIa.: S. Iida, et al.; Clin. Cancer Res. 12, 2879 (2006), Application(s): Flow cytometry analysis of human heparinized peripheral blood, Abstract; Full Text|Triptolide, a constituent of immunosuppressive Chinese herbal medicine, is a potent suppressor of dendritic-cell maturation and trafficking: X. Chen, et al.; Blood 106, 2409 (2005), Application(s): Flow cytometry analysis of human monocytes, Abstract; Full Text|Primary Antibody-Fab Fragment Complexes: A Flexible Alternative to Traditional Direct and Indirect Immunolabeling Techniques: J.K. Brown, et al.; J. Histochem. Cytochem. 52, 1219 (2004), Application(s): IHC on frozen murine jejunum tissue samples, Abstract;|Cytokine Control of Memory B Cell Homing Machinery: M.P. Roy, et al.; J. Immunol. 169, 1676 (2002), Application(s): Flow cytometry analysis of human germinal center B-cells, Abstract; Full Text|Expression of cyclin B1 and cyclin dependent kinase inhibitor p21 in lymphocytes in patients with systemic lupus erythematosus: C.Y. Ho, et al.; J. Rheumatol. 29, 2537 (2002), Abstract;|CD99 Signals Caspase-Independent T Cell Death: R.D. Pettersen, et al.; J. Immunol. 166, 4931 (2001), Abstract; Full Text|Preconditioning with fetal cord blood facilitates engraftment of primary childhood T-cell acute lymphoblastic leukemia in immunodeficient mice: D.P. Dialynas, et al.; Blood 97, 3218 (2001), Application(s): Flow cytometry analysis of human childhood T-lineage acute lymphoblastic leukemia (ALL) cells, Abstract; Full Text|Antigen contacts by Ni-reactive TCR: typical alphass chain cooperation versus alpha chain-dominated specificity: J. Vollmer, et al.; Int. Immunol. 12, 1723 (2000), Application(s): Flow cytometry analysis of human T- and B-cells, Abstract; Full Text|Effect of renal dialysis therapy modality on T cell cytokine production: A. Zamauskaite, et al.; Nephrol. Dial. Transplant. 14, 49 (1999), Application(s): Flow cytometry analysis of human heparinized blood samples, Abstract; Full Text|TCR Reactivity in Human Nickel Allergy Indicates Contacts with Complementarity-Determining Region 3 but Excludes Superantigen-Like Recognition: J. Vollmer, et al.; J. Immunol. 163, 2723 (1999), Application(s): Flow cytometry analysis of human T- and B-cells, Abstract; Full Text