IgG2a, Heavy Chain (APC)

Applications:|Suitable for use in FLISA. Other applications not tested.||Recommended Dilutions:|ELISA: 1.25ug/ml |Optimal working dilutions to be determined by researcher.||Avidity for rat IgG2a: |2.1x10e9 L/mole||Matched Pair:|Capture: I1904-82P - IgG2a, Heavy Chain|Detection: K0100-01B - Kappa, Lambda Light Chain |Standard: I1904-82J1 - IgG2a, Kappa, Myeloma, Rat||Hybridoma:|Spleen cells from immunized BALB/c mice were fused with cells of the mouse PAI-O myeloma cell line.||Recommended Secondary Antibodies:|I1904-06C: IgG, H&L (HRP) (X-Adsorbed) Pab Gt x Mo|I1904-06H: IgG, H&L (HRP) (X-Adsorbed) Pab Gt x Mo|I1904-14C: IgG, Fc (HRP) Pab Gt x Mo|I1904-19C: IgG, F(ab’)2 (HRP) Pab Gt x Mo||Storage and Stability:|Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.||Note: Applications are based on unconjugated antibody.