Specifications

Product Data
Clone Name	E2E3
Applications	ELISA, IHC, WB
Recommended Dilution	ELISA. Immunohistochemistry on formalin-fixed, paraffin-embedded tissues (1-2 µg/ml, incubate at RT for at least 1 hr.). Western blot (1-5 µg/ml).
Reactivities	Bovine, Human, Porcine
Host	Mouse
Isotype	IgG1
Clonality	Monoclonal
Immunogen	Hybridoma produced by the fusion of splenocytes from mice immunized with full-length insulin protein and mouse myeloma cells.
Specificity	This antibody detects Insulin.
Formulation	PBS State: Purified State: Liquid purified Ig fraction Preservative: 0.08% Sodium Azide
Concentration	lot specific
Purification	Affinity Chromatography on Protein A/G
Conjugation	Unconjugated
Storage	Upon receipt, store undiluted (in aliquots) at -20°C. Avoid repeated freezing and thawing.
Stability	Shelf life: one year from despatch.
Gene Name	insulin
Database Link	Entrez Gene 3630 Human UniProt ID P01308
Background	Insulin is one of the major regulatory hormones of intermediate metabolism throughout the body. The biological actions of this hormone involve integration of carbohydrate, protein, and lipid metabolism. Insulin enhances membrane transport of glucose, amino acids, and certain ions. It also promotes glycogen storage, formation of triglycerides and synthesis of proteins and nucleic acids. Immunocytochemical investigations have localized insulin in the B cells of pancreatic islets of Langerhans. Deficiency of insulin results in diabetes mellitus, one of the leading causes of morbidity and mortality in the general population. Insulin is also present in tumors of B cell origin such as insulinoma.
Synonyms	INS
Reference Data