Macerozyme R-10^® can be used in combination with cellulase "Onozuka R-10^®" (cat. no. 16419) (1, 2) or with cellulase "Onozuka RS^®" (cat. no. 16420) for the isolation of plant cells and especially for the isolation of metabolically active protoplasts.

Activity:
Pectinase: approx. 0.5 U/mg; unit definition: 1 unit catalyses the release of 1 µMol of reducing groups from pectic acid per minute at 25 °C, pH 4.5, calculated as galacturonic acid. Reducing groups are determined with an alkaline copper reagent (2).
Hemicellulase: approx. 0.25 U/mg; unit definition: 1 unit corresponds to 1 µMol of reducing groups released from beechwood xylan per hour at 37 °C, pH 5.5, calculated as xylose.
Cellulase: approx. 0.1 U/mg; unit definition: 1 unit catalyses the release of 1 µMol glucose from sodium carboxymethylcellulose per minute at 40 °C, pH 4.5; glucose is determined with an alkaline copper reagent (3).
Optimum pH: 3.5 - 7.0
Optimum temperature: 40 - 50 °C.
Macerozym R-10^® is a registered trademark of Yakult Pharmaceuticals Industry Co., Ltd..


References:

1. Yamada, Y. et al. (1972) Agr. Biol. Chem. 36, 1055-9
2. Barraclough, R. & Ellis, R.J. (1979) Eur. J. Biochem. 94, 165-77
3. Okada, G. (1988) Methods Enzymol. Vol. 160, 259-63

DANGER

Hazard Statements	H334
Precautions	P261 - P285
Reaction	P304 +P341 - P342 +P311

EINECS: 232-885-6 • WGK: 1 • HS: 35079090
Storage Temperature: +2 °C to +8 °C