SH-Sy5y cells
Referencia C0005004
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Disease:
Neuroblastoma Derived from metastatic bone marrow Homo sapiens Brain Epithelial Adherent+suspension Female, 4 yrs of age Ham's F12:AddexBio-formulated EMEM (C0005-01) (1:1) + 15% FBS 1:10 to 1:100 using 0.25% trypsin or trypsin/EDTA, 5% CO2; 37°C Complete culture medium supplemented with 5% (v/v) DMSO I SH-Sy5y cell line is a thrice-cloned sub-line of bone marrow biopsy-derived SK-N-SH. SH-Sy5y has a dopamine-beta-hydroxylase activity and can convert glutamate to GABA. Will form tumors in nude mice in approximately 3-4 weeks. Bacteria: Negative Yeast: Negative Mycoplasma: Negative HIV: Negative Hepatitis B: Negative Hepatitis C: Negative Amelogenin: X; CSF1PO: 11; D13S317: 11; D16S539: 8,13; D5S818: 12; D7S820: 7,10; THO1: 7,10; TPOX: 8,11; vWA: 14,18 If use of this culture results in a scientific publication, it should be cited in the publication as: SH-Sy5y (AddexBio C0005004) B103 LN-319 SH-Sy5y cells U87MG cells Origin:
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References for SH-Sy5y cells:
1. Harris, B., Pereira, I., and Parkin, E. Targeting ADAM10 to lipid rafts in neuroblastoma SH-SY5Y cells impairs amyloidogenic processing of the amyloid precursor protein. Brain Res, 1296: 203-215, 2009.
2. Hettiarachchi, N. T., Parker, A., Dallas, M. L., Pennington, K., Hung, C. C., Pearson, H. A., Boyle, J. P., Robinson, P., and Peers, C. alpha-Synuclein modulation of Ca2+ signaling in human neuroblastoma (SH-SY5Y) cells. J Neurochem, 111: 1192-1201, 2009.
3. Kim, I. S., Koppula, S., Park, P. J., Kim, E. H., Kim, C. G., Choi, W. S., Lee, K. H., and Choi, D. K. Chrysanthemum morifolium Ramat (CM) extract protects human neuroblastoma SH-SY5Y cells against MPP+-induced cytotoxicity. J Ethnopharmacol, 126: 447-454, 2009.
4. Myers, T. A., Kaushal, D., and Philipp, M. T. Microglia are mediators of Borrelia burgdorferi-induced apoptosis in SH-SY5Y neuronal cells. PLoS Pathog, 5: e1000659, 2009.
5. Wu, F., Poon, W. S., Lu, G., Wang, A., Meng, H., Feng, L., Li, Z., and Liu, S. Alpha-synuclein knockdown attenuates MPP+ induced mitochondrial dysfunction of SH-SY5Y cells. Brain Res, 1292: 173-179, 2009.
6. Yang, W., Tiffany-Castiglioni, E., Koh, H. C., and Son, I. H. Paraquat activates the IRE1/ASK1/JNK cascade associated with apoptosis in human neuroblastoma SH-SY5Y cells. Toxicol Lett, 191: 203-210, 2009.
7. McDonald RL, Vanghan PF, Peers C. Muscarinic (M1) receptor-mediated inhibition of K(+)-evoked [3H]-noradrenaline release from human nuuroblastoma (SH-Sy5y) cells via inhibition of L- and N-type Ca2+ channels. Br J Pharmacol 113:459 621-627, 1994.