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> Tissue, Total RNA, Human Adult Normal, Brain, Cerebral Cortex, BioGenomics™

Tissue, Total RNA, Human Adult Normal, Brain, Cerebral Cortex, BioGenomics™

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Referencia T5595-7249-10ug

embalaje : 10ug

Marca : US Biological

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T5595-7249 Tissue, Total RNA, Human Adult Normal, Brain, Cerebral Cortex, BioGenomics™

Clone Type
Polyclonal
Grade
Molecular Biology Grade
Shipping Temp
Dry Ice
Storage Temp
-70°C

Total RNAs are available from almost 200 different human adult and fetal normal tissues, human diseased and tumor tissues, as well as mouse, rat, and monkey tissues. Total RNAs are provided ready-to-use for Northern blotting, cDNA synthesis, RNA protection, and RNA differential display. |Total RNA isolation is performed using proprietary techniques. Contamination by RNase, genomic DNA polysaccharides, and proteoglycans has been effectively eliminated. The integrity of the total RNAs is assured by checking for a ratio of greater than 1/1 between 28S and 18S ribosomal RNA. We guarantee high efficiency reverse transcription.||Applications:|Northern Blot, RT-PCR, RACE, |cDNA synthesis, cDNA library construction, cDNA probe for profiling study in gene expression, RNA protection and primer extension, RNA display, Purification of mRNA||||||||

Applications
Features:|• Total RNA isolated from a wide variety of hard to obtain tissues |• Decontamination of polysaccharide, proteoglycan, RNase, and genomic DNA |• Extensive quality control procedures to ensure high quality |• High efficiency reverse transcription |• Available documentation of tissues' clinical histories (additional cost).||Purity:|Total RNA isolated by modified guanidine thiocyanate techniques.||Form:|Supplied as a liquid in RNA storage buffer.||Concentration:|2.39mg/ml||Donor Information:|RNA from one 26-year old normal, male, adult human.|Quality Control:|1. The integrity of the RNA is examined by visual inspection for the presence of intact bands of 18S and 28S ribosomal RNA when electrophoreses on a denaturing agarose gel. The quality and puiryt of total RNA were tested by spectrophotometer. A260/A280 is 1.8-2.0 (detected in 10mM Tris-Cl, pH 7.5).|2. The RNA is treated by DNase I. It is tested as DNA free RNA by PCR.|3. cDNA synthesis is successfully performed by using this RNA as template. ||Storage and Stability:|For long-term storage, aliquot to avoid repeated freezing and thawing and freeze at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquots are stable for 6 months.
Form
Supplied as a liquid in RNA Storage Buffer: 0.1% DEPC-treated water with 0.1mM EDTA. No non-specific endonuleases, no nickase, no exonuclease and no RNase detected.