AP8571a-ev | TOB1 Antibody (N-term) Clinisciences

Western blot analysis of TOB1 Antibody (N-term) (Cat. #AP8571a) in mouse liver tissue lysates (35ug/lane). TOB1 (arrow) was detected using the purified Pab.
Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with TOB1 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
TOB1 Antibody (N-term) (Cat. #AP8571a) flow cytometric analysis of NCI-H292 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Product Information
Application Applications Legend: WB=Western Blot IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin-embedded Sections) IHC-F=Immunohistochemistry (Frozen Sections) IF=Immunofluorescence FC=Flow Cytopmetry IC=Immunochemistry ICC=Immunocytochemistry E=ELISA IP=Immunoprecipitation DB=Dot Blot CHIP=Chromatin Immunoprecipitation FA=Fluorescence Assay IEM=Immuno electron microscopy EIA=Enzyme Immunoassay	FC, IHC-P, WB, E
Primary Accession	P50616
Other Accession	Q8R5K6, Q61471
Reactivity	Human, Mouse
Predicted	Rat
Host	Rabbit
Clonality	Polyclonal
Isotype	Rabbit IgG
Calculated MW	38155 Da
Antigen Region	54-83 aa

Additional Information
Gene ID	10140
Other Names	Protein Tob1, Transducer of erbB-2 1, TOB1, TOB, TROB1
Target/Specificity	This TOB1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 54-83 amino acids from the N-terminal region of human TOB1.
Dilution	WB~~1:1000 IHC-P~~1:50~100 FC~~1:10~50
Format	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautions	TOB1 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.

Protein Information
Name	TOB1
Synonyms	TOB, TROB1
Function	Anti-proliferative protein; the function is mediated by association with deadenylase subunits of the CCR4-NOT complex (PubMed:23236473, PubMed:8632892). Mediates CPEB3-accelerated mRNA deadenylation by binding to CPEB3 and recruiting CNOT7 which leads to target mRNA deadenylation and decay (PubMed:21336257).
Cellular Location	Cytoplasm. Nucleus. Note=Only a small fraction localizes to the cytoplasm except in late S- phase where more than half of proteins become cytoplasmic
Tissue Location	Ubiquitous.

TOB1 Antibody (N-term)