UGP2 Rabbit Polyclonal Antibody
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Specifications
Product Data | |
Applications | ELISA, ID, IF, IP, R, WB |
Recommended Dilution | This product is intended for use in precipitating and non-precipitating antibody-binding assays (such as e.g., ELISA and Western blotting and Immunofluorescence or Histochemical techniques), to prepare an insoluble immuno-affinity adsorbent, for labelling with a marker of choice. Working Dilutions: Non-precipitating antibody-binding techniques: 1/1,000-1/7,000. |
Reactivities | Bovine |
Host | Rabbit |
Isotype | IgG |
Clonality | Polyclonal |
Immunogen | Uridine-5’-diphosphoglucose Pyrophosphorylase isolated and purified from Bovine liver. Freund’s complete adjuvant is used in the first step of the immunization procedure. |
Specificity | The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: Immunoelectrophoresis, Cross-Immunoelectrophoresis, single Radial Immunodiffusion (Ouchterlony), block titration, ELISA, Immunoblotting and Enzyme Inhibition. Cross-reactivities against enzymes of other sources may occur but have not been determined. |
Formulation | PBS, pH 7.2 without preservatives and foreign proteins State: Azide Free State: Lyophilized hyperimmune IgG fraction |
Reconstitution Method | Restore by adding 1.0 ml of sterile distilled water |
Concentration | lot specific |
Purification | Ammonium Sulphate Precipitation and Ion Exchange Chromatography |
Conjugation | Unconjugated |
Storage | Store the antibody lyophilized at 2-8°C and reconstituted at 2-8°C for one week or (in aliquots) at -20°C for longer. If a slight precipitation occurs upon storage, this should be removed by centrifugation. |
Stability | Shelf life: one year from despatch. |
Database Link | |
Background | UGP2 is an important intermediary in mammalian carbohydrate interconversions. It transfers a glucose moiety from glucose-1-phosphate to MgUTP and forms UDP-glucose and MgPPi. In liver and muscle tissue,UDP-glucose is a direct precursor of glycogen; in lactating mammary gland it is converted to UDP-galactose which is then converted to lactose. The eukaryotic enzyme has no significant sequence similarity to the prokaryotic enzyme. |
Synonyms | UGP1, UDP-glucose pyrophosphorylase, UGPase, UDPGP |
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